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dc.contributor.authorMaldonado-Barragán, A.
dc.contributor.authorAlegría-Carrasco, E.
dc.contributor.authorBlanco, M.M.
dc.contributor.authorVela, A.I.
dc.contributor.authorFernández-Garayzábal, J.F.
dc.contributor.authorRodríguez, J.M.
dc.contributor.authorGibello, A.
dc.identifier.citationMaldonado-Barragán , A , Alegría-Carrasco , E , Blanco , M M , Vela , A I , Fernández-Garayzábal , J F , Rodríguez , J M & Gibello , A 2022 , ' Garvicins AG1 and AG2 : two novel class IId bacteriocins of lactococcus garvieae Lg-Granada ' , International Journal of Molecular Sciences , vol. 23 , no. 9 , 4685 .
dc.identifier.otherPURE: 279430099
dc.identifier.otherPURE UUID: 1f4a2e44-e88b-4a10-9d39-95560eeb1330
dc.identifier.otherRIS: urn:C40FF59E61E9EF81801210496DB2489A
dc.identifier.otherORCID: /0000-0002-4775-346X/work/112711659
dc.identifier.otherWOS: 000794507200001
dc.identifier.otherScopus: 85128720340
dc.descriptionFunding: This research was funded by the Spanish Ministry of Science, Innovation, and Universities, grant number RTI2018-098530-B-I00. The APC was funded by the Spanish Ministry of Science, Innovation, and Universities, grant number RTI2018-098530-B-I00.en
dc.description.abstractLactococcus garvieae causes infectious diseases in animals and is considered an emerging zoonotic pathogen involved in human clinical conditions. In silico analysis of plasmid pLG50 of L. garvieae Lg-Granada, an isolate from a patient with endocarditis, revealed the presence of two gene clusters (orf 46–47 and orf 48–49), each one encoding a novel putative bacteriocin, i.e., garvicin AG1 (GarAG1; orf 46) and garvicin AG2 (GarAG2; orf 48), and their corresponding immunity proteins (orf 47 and orf 49). The chemically synthesised bacteriocins GarAG1 and GarAG2 presented inhibitory activity against pathogenic L. garvieae strains, with AG2 also being active against Listeria monocytogenes, Listeria ivanovii and Enterococcus faecalis. Genetic organisation, amino acid sequences and antimicrobial activities of GarAG1 and GarAG2 indicate that they belong to linear non-pediocin-like one-peptide class IId bacteriocins. Gram-positive bacteria that were sensitive to GarAG2 were also able to ferment mannose, suggesting that this bacteriocin could use the mannose phosphotransferase transport system (Man-PTS) involved in mannose uptake as a receptor in sensitive strains. Intriguingly, GarAG1 and GarAG2 were highly active against their own host, L. garvieae Lg-Granada, which could be envisaged as a new strategy to combat pathogens via their own weapons.
dc.relation.ispartofInternational Journal of Molecular Sciencesen
dc.rightsCopyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).en
dc.subjectAntimicrobial peptidesen
dc.subjectIn silico analysisen
dc.subjectZoonotic diseasesen
dc.subjectQR Microbiologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleGarvicins AG1 and AG2 : two novel class IId bacteriocins of lactococcus garvieae Lg-Granadaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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