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dc.contributor.authorKumar, Pankaj
dc.contributor.authorBoyne, Caitlin
dc.contributor.authorBrown, Sydney Erin
dc.contributor.authorQureshi, Ayesha
dc.contributor.authorThorpe, Peter Jacob
dc.contributor.authorSynowsky, Silvia Anna
dc.contributor.authorShirran, Sally Lorna
dc.contributor.authorPowis, Simon John
dc.identifier.citationKumar , P , Boyne , C , Brown , S E , Qureshi , A , Thorpe , P J , Synowsky , S A , Shirran , S L & Powis , S J 2022 , ' Tumour-associated antigenic peptides are present in the HLA class I ligandome of cancer cell line derived extracellular vesicles ' , Immunology , vol. 166 , no. 2 , 13471 , pp. 249-264 .
dc.identifier.otherPURE: 278370124
dc.identifier.otherPURE UUID: 4cb52635-4ea5-4c66-a989-09ff1904086a
dc.identifier.otherORCID: /0000-0003-4218-2984/work/111970908
dc.identifier.otherORCID: /0000-0003-3516-3507/work/111971496
dc.identifier.otherWOS: 000789523400001
dc.identifier.otherScopus: 85128414017
dc.descriptionFunding: Breast Cancer Now (Grant Number(s): 2018JulPR1086), Wellcome Trust (GrantNumber(s): 105621/Z/14/Z), Melville Charitable Trust.en
dc.description.abstractThe recent success of monoclonal antibody checkpoint inhibitor therapies that enhance the ability of CD8+ T cells to detect cancer-related antigenic peptides has refocused the need to fully understand the repertoire of peptides being presented to the immune system. Whilst the peptide ligandome presented by cell surface human leucocyte antigen class I (HLA-I) molecules on cancer cells has been studied extensively, the ligandome of extracellular vesicles (EVs) remains poorly defined. Here we report the HLA-I ligandome of both the cell surface and EVs from eight breast cancer cell lines (MCF7, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-453, HCC 1806, HCC 1395, and HCC 1954), and additionally the melanoma cell line ESTDAB-056 and the multiple myeloma line RPMI 8226. Utilising HLA-I immunoisolation and mass spectrometry, we detected a total of 6574 peptides from the cell surface and 2461 peptides from the EVs of the cell lines studied. Within the EV HLA-I ligandome, we identified 150 peptides derived from tumour associated antigenic proteins, of which 19 peptides have been shown to elicit T cell responses in previous studies. Our data thus shows the prevalence of clinically relevant tumour-associated antigenic peptides in the HLA-I ligandome presented on EV.
dc.rightsCopyright © 2022 The Authors. Immunology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectExtracellular vesiclesen
dc.subjectTumour associated antigen (TAA)en
dc.subjectHLA ligandomeen
dc.subjectT-cell epitopesen
dc.subjectBreast canceren
dc.subjectQR180 Immunologyen
dc.titleTumour-associated antigenic peptides are present in the HLA class I ligandome of cancer cell line derived extracellular vesiclesen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.description.statusPeer revieweden

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