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dc.contributor.authorMusisi, Emmanuel
dc.contributor.authorSessolo, Abdul
dc.contributor.authorKaswabuli, Sylvia
dc.contributor.authorZawedde, Josephine
dc.contributor.authorByanyima, Patrick
dc.contributor.authorKasiinga, Shariifah
dc.contributor.authorSanyu, Ingvar
dc.contributor.authorUwimaana, Esther
dc.contributor.authorWalimbwa, Stanley
dc.contributor.authorOla, Joseph
dc.contributor.authorSsengooba, Willy
dc.contributor.authorSekaggya, Christine
dc.contributor.authorJoloba, Moses L.
dc.contributor.authorWorodria, William
dc.contributor.authorHuang, Laurence
dc.contributor.authorGillespie, Stephen Henry
dc.contributor.authorSloan, Derek James
dc.contributor.authorSabiiti, Wilber
dc.date.accessioned2022-01-13T13:30:11Z
dc.date.available2022-01-13T13:30:11Z
dc.date.issued2022-02-23
dc.identifier.citationMusisi , E , Sessolo , A , Kaswabuli , S , Zawedde , J , Byanyima , P , Kasiinga , S , Sanyu , I , Uwimaana , E , Walimbwa , S , Ola , J , Ssengooba , W , Sekaggya , C , Joloba , M L , Worodria , W , Huang , L , Gillespie , S H , Sloan , D J & Sabiiti , W 2022 , ' High Mycobacterium tuberculosis bacillary loads detected by tuberculosis molecular bacterial load assay in patient stool : a potential alternative for nonsputum diagnosis and treatment response monitoring of tuberculosis ' , Microbiology Spectrum , vol. 10 , no. 1 , e02100-21 . https://doi.org/10.1128/spectrum.02100-21en
dc.identifier.issn2165-0497
dc.identifier.otherPURE: 277100107
dc.identifier.otherPURE UUID: e2187124-3233-4001-bd89-03830a8349a4
dc.identifier.otherPubMed: 35019686
dc.identifier.otherORCID: /0000-0001-6537-7712/work/106397450
dc.identifier.otherORCID: /0000-0002-4742-2791/work/106397574
dc.identifier.otherORCID: /0000-0002-7888-5449/work/106397813
dc.identifier.otherScopus: 85124281960
dc.identifier.otherWOS: 000766015800180
dc.identifier.urihttp://hdl.handle.net/10023/24655
dc.descriptionFunding: Emmanuel’s doctoral research is supported by European and Developing countries Clinical Trial Partnership (EDCTP)-funded PanACEA II studentship (TR1A2015-1102), and the University of St Andrews St Leonards scholarship. Funding from the Infectious Diseases Institute, Makerere University to Mr Emmanuel Musisi and Dr Abdul Sessolo through Health and Innovation Impact project supported collection of specimens. Enrolment was funded by the Lung MicroCHIP (NIH: U01 HL098964) and K24 (NIH: K24 HL087713) grants through Professor Laurence Huang. Funding from the Scottish Funding Council (SCF)-Global401 Challenges Research Fund (GCRF) supported the TB-MBLA processing of the samples.en
dc.description.abstractNot all patients produce sputum, yet most available TB tests use sputum. We investigated the utility of a novel RNA-based quantitative test, the tuberculosis molecular bacterial load assay (TB-MBLA), for the detection and quantification of Mycobacterium tuberculosis in stool. Stools from 100 adult individuals were treated with OMNIgene-sputum reagent and tested using Xpert MTB/RIF ultra (Xpert ultra), auramine O smear microscopy (smear), mycobacterial growth indicator tube (MGIT), and Lowenstein-Jensen (LJ) cultures. The remaining portions were frozen at −20°C and later tested by TB-MBLA. MGIT sputum culture was used as a TB confirmatory test and reference for stool tests. Sixty-one of 100 participants were already confirmed TB positive by MGIT sputum culture, 20 (33%) of whom were HIV coinfected. TB-MBLA detected M. tuberculosis in 57/100 stool samples, including 49 already confirmed for TB. The mean bacterial load measured by stool TB-MBLA was 5.67 ± 1.7 log10 estimated CFU (eCFU) per mL in HIV-coinfected participants, which was higher than the 4.83 ± 1.59 log10 eCFU per mL among the HIV-negative participants (P = 0.04). The sensitivities (95% confidence intervals [CI]) of stool assays were 80% (68 to 89) and 90% (79 to 98) for TB-MBLA and Xpert ultra, which were both higher than the 44% (32 to 58), 64% (51 to 76), and 62% (45 to 77) for smear, MGIT, and Lowenstein-Jensen (LJ) stool cultures, respectively. The specificity (95% CI) of stool assays was highest for smear, at 97% (87 to 100), followed by Xpert ultra at 91% (76 to 98), TB-MBLA at 79% (63 to 90), LJ at 80% (64 to 91), and MGIT at 62% (45 to 77). Twenty-six percent of MGIT and 21% of LJ stool cultures were indeterminate due to contamination. Detection and quantification of viable M. tuberculosis bacilli in stool raises its utility as an alternative to sputum as a sample type for TB diagnosis.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofMicrobiology Spectrumen
dc.rightsCopyright © 2022 Musisi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.subjectMolecular bacterial load assayen
dc.subjectMolecular diagnosticsen
dc.subjectMycobacterium tuberculosisen
dc.subjectQR Microbiologyen
dc.subjectNDASen
dc.subject.lccQRen
dc.titleHigh Mycobacterium tuberculosis bacillary loads detected by tuberculosis molecular bacterial load assay in patient stool : a potential alternative for nonsputum diagnosis and treatment response monitoring of tuberculosisen
dc.typeJournal articleen
dc.contributor.sponsorScottish Funding Councilen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1128/spectrum.02100-21
dc.description.statusPeer revieweden
dc.identifier.grantnumberN/Aen


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