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Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

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dc.contributor.authorBossé, Janine T.
dc.contributor.authorLi, Yanwen
dc.contributor.authorCohen, Liza Miriam
dc.contributor.authorStegger, Marc
dc.contributor.authorAngen, Øystein
dc.contributor.authorLacouture, Sonia
dc.contributor.authorGottschalk, Marcelo
dc.contributor.authorLei, Liancheng
dc.contributor.authorKoene, Miriam
dc.contributor.authorKuhnert, Peter
dc.contributor.authorBandara, Aloka B.
dc.contributor.authorInzana, Thomas J.
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorHarris, David
dc.contributor.authorOshota, Olusegun
dc.contributor.authorMaskell, Duncan J.
dc.contributor.authorTucker, Alexander W.
dc.contributor.authorWren, Brendan W.
dc.contributor.authorRycroft, Andrew N.
dc.contributor.authorLangford, Paul R.
dc.contributor.authorBRaDP1T Consortium
dc.date.accessioned2021-12-07T13:30:08Z
dc.date.available2021-12-07T13:30:08Z
dc.date.issued2021-11-24
dc.identifier.citationBossé , J T , Li , Y , Cohen , L M , Stegger , M , Angen , Ø , Lacouture , S , Gottschalk , M , Lei , L , Koene , M , Kuhnert , P , Bandara , A B , Inzana , T J , Holden , M T G , Harris , D , Oshota , O , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R & BRaDP1T Consortium 2021 , ' Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation ' , Microbial Genomics , vol. 7 , no. 11 , 687 . https://doi.org/10.1099/mgen.0.000687en
dc.identifier.issn2057-5858
dc.identifier.otherPURE: 277002182
dc.identifier.otherPURE UUID: 7ab8b890-360c-49c2-b496-262ac3271f7c
dc.identifier.otherJisc: 49b5fde573334aa29940b113757c5a38
dc.identifier.otherORCID: /0000-0002-4958-2166/work/104619072
dc.identifier.otherScopus: 85122168835
dc.identifier.urihttp://hdl.handle.net/10023/24483
dc.descriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the ‘National Natural Science Foundation of China’ (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051).en
dc.description.abstractWe report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofMicrobial Genomicsen
dc.rightsCopyright © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.en
dc.subjectSerovar 8 reference genomeen
dc.subjectSNPsen
dc.subjectActinobacillus pleuropneumoniaeen
dc.subjectQH426 Geneticsen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQH426en
dc.subject.lccQRen
dc.titleComplete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.identifier.doihttps://doi.org/10.1099/mgen.0.000687
dc.description.statusPeer revieweden


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