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dc.contributor.authorBentley, Kirsten
dc.contributor.authorTee, Han Kang
dc.contributor.authorPearson, Ashley
dc.contributor.authorLowry, Kym
dc.contributor.authorWaugh, Sheila
dc.contributor.authorJones, Sian
dc.contributor.authorChan, Yoke Fun
dc.contributor.authorEvans, David John
dc.date.accessioned2021-11-29T15:30:07Z
dc.date.available2021-11-29T15:30:07Z
dc.date.issued2021-11-29
dc.identifier.citationBentley , K , Tee , H K , Pearson , A , Lowry , K , Waugh , S , Jones , S , Chan , Y F & Evans , D J 2021 , ' Isolation and identification of inter-species Enterovirus recombinant genomes ' , Viruses , vol. 13 , no. 12 , 2390 . https://doi.org/10.3390/v13122390en
dc.identifier.issn1999-4915
dc.identifier.otherPURE: 276859610
dc.identifier.otherPURE UUID: 17c1289e-5b8e-46df-b4cc-d9b7ab24ee7d
dc.identifier.otherORCID: /0000-0002-1315-4258/work/104252541
dc.identifier.otherORCID: /0000-0002-6619-2098/work/104252619
dc.identifier.otherORCID: /0000-0003-4183-985X/work/104252854
dc.identifier.otherScopus: 85120793620
dc.identifier.otherWOS: 000745294400001
dc.identifier.urihttps://hdl.handle.net/10023/24427
dc.descriptionThis research was funded by the Biotechnology and Biological Sciences Research Council (BB/M009343/1 to D.J.E), an ISSF award from The Wellcome Trust to the BSRC, University of St Andrews, and a Royal Society Newton Advanced Fellowship (NA160353 to Y.F.C). The APC was funded by The University of St Andrews.en
dc.description.abstractPositive-strand RNA virus evolution is partly attributed to the process of recombination. Although common between closely genetically related viruses, such as within species of the Enterovirus genus of the Picornaviridae family, inter-species recombination is rarely observed in nature. Recent studies have shown recombination is a ubiquitous process resulting in a wide range of recombinant genomes and progeny viruses. While not all recombinant genomes yield infectious virus progeny, their existence and continued evolution during replication has critical implications for evolution of the virus population. In this study we utilised an in vitro recombination assay to demonstrate inter-species recombination events between viruses from four enterovirus species’, A-D. We show that inter-species recombinant genomes are generated in vitro with polymerase template-switching events occurring within the virus polyprotein coding region. However, these genomes did not yield infectious progeny virus. Analysis and attempted recovery of a constructed recombinant cDNA revealed a restriction in positive- but not negative -strand RNA synthesis, indicating a significant block in replication. This study demonstrates the propensity for inter-species recombination at the genome level but suggests that significant sequence plasticity would be required in order to overcome blocks in the virus life cycle and allow for the production of infectious virus.
dc.format.extent17
dc.language.isoeng
dc.relation.ispartofVirusesen
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/).en
dc.subjectRecombinationen
dc.subjectEnterovirusen
dc.subjectVirus evolutionen
dc.subjectQR355 Virologyen
dc.subjectNDASen
dc.subject.lccQR355en
dc.titleIsolation and identification of inter-species Enterovirus recombinant genomesen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorBBSRCen
dc.contributor.sponsorThe Royal Societyen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.3390/v13122390
dc.description.statusPeer revieweden
dc.identifier.urlhttps://www.mdpi.com/1999-4915/13/12/2390en
dc.identifier.grantnumberen
dc.identifier.grantnumberBB/M009343/1en
dc.identifier.grantnumberNA160353en


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