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dc.contributor.authorGómez, Marta
dc.contributor.authorValverde, Arancha
dc.contributor.authordel Campo, Rosa
dc.contributor.authorRodríguez, Juan Miguel
dc.contributor.authorMaldonado-Barragán, Antonio
dc.identifier.citationGómez , M , Valverde , A , del Campo , R , Rodríguez , J M & Maldonado-Barragán , A 2021 , ' Phenotypic and molecular characterization of commensal, community-acquired and nosocomial Klebsiella spp. ' , Microorganisms , vol. 9 , no. 11 , e2344 .
dc.identifier.otherPURE: 276713904
dc.identifier.otherPURE UUID: ac314b9e-e2b1-4a93-baa8-52aa06ef114e
dc.identifier.otherJisc: d8819da95c84446684201bf05ae5b185
dc.identifier.otherORCID: /0000-0002-4775-346X/work/103511249
dc.identifier.otherScopus: 85118878656
dc.identifier.otherWOS: 000726026000001
dc.descriptionThis research was funded by Ministerio de Ciencia e Innovación (Spain), grant numbers CSD2007-00063 (FUN-C-FOOD, Consolider-Ingenio 2010), AGL2010-15420 and PID2019-105606RB-I00.en
dc.description.abstractKlebsiella spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal Klebsiella spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the rpoB gene was useful for species identification (K. pneumoniae, K. variicola, K. quasipneumoniae, K. oxytoca, K. grimontii, K. michiganensis, Raoultella planticola and R. ornithinolytica), previously misidentified as K. pneumoniae or K. oxytoca by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR K. quasipneumoniae isolate was also found. A commensal K. pneumoniae strain showed a potent bacteriocin-like inhibitory activity against MDR Klebsiella isolates, thus highlighting the potential role of commensal Klebsiella spp. in health and disease.
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://
dc.subjectAntibiotic resistanceen
dc.subjectQR Microbiologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titlePhenotypic and molecular characterization of commensal, community-acquired and nosocomial Klebsiella spp.en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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