Files in this item
Monitoring RNA dynamics in native transcriptional complexes
Item metadata
dc.contributor.author | Chauvier, Adrien | |
dc.contributor.author | St-Pierre, Patrick | |
dc.contributor.author | Nadon, Jean-Francois | |
dc.contributor.author | Hien, Elsa | |
dc.contributor.author | Perez Gonzalez, Daniel Cibran | |
dc.contributor.author | Eschbach, Sebastien H. | |
dc.contributor.author | Lamontagne, Anne-Marie | |
dc.contributor.author | Penedo , Carlos | |
dc.contributor.author | Lafontaine, Daniel A. | |
dc.date.accessioned | 2021-11-09T11:30:09Z | |
dc.date.available | 2021-11-09T11:30:09Z | |
dc.date.issued | 2021-11-09 | |
dc.identifier | 275982808 | |
dc.identifier | 7eaff25f-9e0e-4136-b584-8681cf70ab11 | |
dc.identifier | 85119264028 | |
dc.identifier | 000720928400009 | |
dc.identifier.citation | Chauvier , A , St-Pierre , P , Nadon , J-F , Hien , E , Perez Gonzalez , D C , Eschbach , S H , Lamontagne , A-M , Penedo , C & Lafontaine , D A 2021 , ' Monitoring RNA dynamics in native transcriptional complexes ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 118 , no. 45 , e2116155118 . https://doi.org/10.1073/pnas.2106564118 | en |
dc.identifier.issn | 0027-8424 | |
dc.identifier.other | ORCID: /0000-0002-5807-5385/work/103137322 | |
dc.identifier.uri | https://hdl.handle.net/10023/24298 | |
dc.description | This work was supported by grants from the Canadian Institutes of Health Research, the Natural Sciences and Engineering Research Council of Canada. JCP wishes to thank the Scottish Universities Physics Alliance (SUPA) and the Engineering and Physical Sciences Research Council for support. C. P. G. thanks EPSRC and the University of St Andrews for a PhD scholarship. | en |
dc.description.abstract | Cotranscriptional RNA folding is crucial for the timely control of biological processes, but because of its transient nature, its study has remained challenging. While single-molecule Förster resonance energy transfer (smFRET) is unique to investigate transient RNA structures, its application to cotranscriptional studies has been limited to nonnative systems lacking RNA polymerase (RNAP)–dependent features, which are crucial for gene regulation. Here, we present an approach that enables site-specific labeling and smFRET studies of kilobase-length transcripts within native bacterial complexes. By monitoring Escherichia coli nascent riboswitches, we reveal an inverse relationship between elongation speed and metabolite-sensing efficiency and show that pause sites upstream of the translation start codon delimit a sequence hotspot for metabolite sensing during transcription. Furthermore, we demonstrate a crucial role of the bacterial RNAP actively delaying the formation, within the hotspot sequence, of competing structures precluding metabolite binding. Our approach allows the investigation of cotranscriptional regulatory mechanisms in bacterial and eukaryotic elongation complexes. | |
dc.format.extent | 11 | |
dc.format.extent | 2860064 | |
dc.language.iso | eng | |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | en |
dc.subject | Single-molecule FRET | en |
dc.subject | Transcription | en |
dc.subject | RNA | en |
dc.subject | Riboswitch | en |
dc.subject | QH301 Biology | en |
dc.subject | QH426 Genetics | en |
dc.subject | NDAS | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QH426 | en |
dc.title | Monitoring RNA dynamics in native transcriptional complexes | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.identifier.doi | https://doi.org/10.1073/pnas.2106564118 | |
dc.description.status | Peer reviewed | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.