Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorOsei-Wusu, Stephen
dc.contributor.authorMorgan, Portia
dc.contributor.authorAsare, Prince
dc.contributor.authorAdams, Godfrey
dc.contributor.authorMusah, Abdul B.
dc.contributor.authorSiam, Ishaque M.
dc.contributor.authorGillespie, Stephen Henry
dc.contributor.authorSabiiti, Wilber
dc.contributor.authorYeboah-Manu, Dorothy
dc.date.accessioned2021-10-27T08:30:02Z
dc.date.available2021-10-27T08:30:02Z
dc.date.issued2021-10-27
dc.identifier.citationOsei-Wusu , S , Morgan , P , Asare , P , Adams , G , Musah , A B , Siam , I M , Gillespie , S H , Sabiiti , W & Yeboah-Manu , D 2021 , ' Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa ' , Frontiers in Microbiology , vol. 12 , 719531 . https://doi.org/10.3389/fmicb.2021.719531en
dc.identifier.issn1664-302X
dc.identifier.otherPURE: 276265540
dc.identifier.otherPURE UUID: 2a837711-de51-4f15-876a-bdfabd7c531e
dc.identifier.otherORCID: /0000-0001-6537-7712/work/102330484
dc.identifier.otherORCID: /0000-0002-4742-2791/work/102330560
dc.identifier.otherScopus: 85118801269
dc.identifier.otherWOS: 000717639700001
dc.identifier.urihttps://hdl.handle.net/10023/24194
dc.descriptionFunding: This work was funded by The Royal Society Africa Prize 2018 awarded to Dorothy Yeboah-Manu and supported by funding from Scottish Funding Council (SCF)-Global Challenges Research Fund (GCRF) to Prof Stephen Gillespie and Dr Wilber Sabiiti.en
dc.description.abstractStudies have shown an association between bacterial load and virulence; however, not much is known about the diversity in this phenotypic characteristic of Mycobacterium tuberculosis complex (MTBC). This study was therefore aimed to determine the differences in bacterial load of the three most prevalent MTBC genotypes (L4, L5 and L6) in West Africa at the time of diagnosis. A total of 170 paired fresh sputum samples were collected; one part in guanidinium thiocyanate (GTC) was used for RNA extraction and tuberculosis Molecular Bacterial Load Assay (TB-MBLA) and the other part without GTC was confirmed for TB positivity using Gene Xpert MTB/RIF, smear microscopy grading and culture on Löwenstein-Jensen media slants. The 170 sputum samples comprised of 155 new cases, 3 follow-up cases and 12 TB negative sputum samples. The time-to-culture-positivity (TTP) and degree of culture positivity (DCP) were recorded. All 122 isolates obtained were Spoligotyped for lineage (L) classification but spoligotypes were obtained from 120 isolates. Of the typed isolates, 70.0%, 10.8%, 10.8%, 4.2%, 2.5%, 0.8% and 0.8% were Lineage- 4, 5, 6, 2, 3, 1, and M. bovis respectively. Further analysis of the three most prevalent lineages showed significantly shorter TTP and higher DCP by L4 compared to L5 and L6 respectively: TTP 20.8, versus 26.5, and 28.2 days; p-value=0.005 and DCP 1.27, versus 0.81 and 0.29, p<0.001. Average TB-MBLA measured bacterial load of L4 was 3.82 Log10eCFU/mL which was not significantly different from 3.81 and 3.80 Log10eCFU/mL of L5 and L6 respectively, p=0.84. Degree of smear microscopy: L4=1.20, L5=1.20, L6=0.92 and Gene Xpert Cq values: L4=17.08, L5=18.37, L6=17.59; showed no significant difference between the lineages, p=0.72 and p=0.48 respectively. Retrospective analysis of a larger sample confirmed the difference in TTP, p<0.001. In conclusion, the observed shorter TTP and high DCP of L4 could signify high growth rate in culture that is independent of total bacterial load at diagnosis.
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiologyen
dc.rightsCopyright © 2021 Osei-Wusu, Morgan, Asare, Adams, Musah, Siam, Gillespie, Sabiiti and Yeboah-Manu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectWest Africaen
dc.subjectMycobacterium africanumen
dc.subjectBacterial loaden
dc.subjectMycobacterium tuberculosisen
dc.subjectTB/MBLAen
dc.subjectQR Microbiologyen
dc.subjectRC Internal medicineen
dc.subjectNDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQRen
dc.subject.lccRCen
dc.titleBacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.719531
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record