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Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa
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dc.contributor.author | Osei-Wusu, Stephen | |
dc.contributor.author | Morgan, Portia | |
dc.contributor.author | Asare, Prince | |
dc.contributor.author | Adams, Godfrey | |
dc.contributor.author | Musah, Abdul B. | |
dc.contributor.author | Siam, Ishaque M. | |
dc.contributor.author | Gillespie, Stephen Henry | |
dc.contributor.author | Sabiiti, Wilber | |
dc.contributor.author | Yeboah-Manu, Dorothy | |
dc.date.accessioned | 2021-10-27T08:30:02Z | |
dc.date.available | 2021-10-27T08:30:02Z | |
dc.date.issued | 2021-10-27 | |
dc.identifier.citation | Osei-Wusu , S , Morgan , P , Asare , P , Adams , G , Musah , A B , Siam , I M , Gillespie , S H , Sabiiti , W & Yeboah-Manu , D 2021 , ' Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa ' , Frontiers in Microbiology , vol. 12 , 719531 . https://doi.org/10.3389/fmicb.2021.719531 | en |
dc.identifier.issn | 1664-302X | |
dc.identifier.other | PURE: 276265540 | |
dc.identifier.other | PURE UUID: 2a837711-de51-4f15-876a-bdfabd7c531e | |
dc.identifier.other | ORCID: /0000-0001-6537-7712/work/102330484 | |
dc.identifier.other | ORCID: /0000-0002-4742-2791/work/102330560 | |
dc.identifier.other | Scopus: 85118801269 | |
dc.identifier.other | WOS: 000717639700001 | |
dc.identifier.uri | https://hdl.handle.net/10023/24194 | |
dc.description | Funding: This work was funded by The Royal Society Africa Prize 2018 awarded to Dorothy Yeboah-Manu and supported by funding from Scottish Funding Council (SCF)-Global Challenges Research Fund (GCRF) to Prof Stephen Gillespie and Dr Wilber Sabiiti. | en |
dc.description.abstract | Studies have shown an association between bacterial load and virulence; however, not much is known about the diversity in this phenotypic characteristic of Mycobacterium tuberculosis complex (MTBC). This study was therefore aimed to determine the differences in bacterial load of the three most prevalent MTBC genotypes (L4, L5 and L6) in West Africa at the time of diagnosis. A total of 170 paired fresh sputum samples were collected; one part in guanidinium thiocyanate (GTC) was used for RNA extraction and tuberculosis Molecular Bacterial Load Assay (TB-MBLA) and the other part without GTC was confirmed for TB positivity using Gene Xpert MTB/RIF, smear microscopy grading and culture on Löwenstein-Jensen media slants. The 170 sputum samples comprised of 155 new cases, 3 follow-up cases and 12 TB negative sputum samples. The time-to-culture-positivity (TTP) and degree of culture positivity (DCP) were recorded. All 122 isolates obtained were Spoligotyped for lineage (L) classification but spoligotypes were obtained from 120 isolates. Of the typed isolates, 70.0%, 10.8%, 10.8%, 4.2%, 2.5%, 0.8% and 0.8% were Lineage- 4, 5, 6, 2, 3, 1, and M. bovis respectively. Further analysis of the three most prevalent lineages showed significantly shorter TTP and higher DCP by L4 compared to L5 and L6 respectively: TTP 20.8, versus 26.5, and 28.2 days; p-value=0.005 and DCP 1.27, versus 0.81 and 0.29, p<0.001. Average TB-MBLA measured bacterial load of L4 was 3.82 Log10eCFU/mL which was not significantly different from 3.81 and 3.80 Log10eCFU/mL of L5 and L6 respectively, p=0.84. Degree of smear microscopy: L4=1.20, L5=1.20, L6=0.92 and Gene Xpert Cq values: L4=17.08, L5=18.37, L6=17.59; showed no significant difference between the lineages, p=0.72 and p=0.48 respectively. Retrospective analysis of a larger sample confirmed the difference in TTP, p<0.001. In conclusion, the observed shorter TTP and high DCP of L4 could signify high growth rate in culture that is independent of total bacterial load at diagnosis. | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Microbiology | en |
dc.rights | Copyright © 2021 Osei-Wusu, Morgan, Asare, Adams, Musah, Siam, Gillespie, Sabiiti and Yeboah-Manu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en |
dc.subject | West Africa | en |
dc.subject | Mycobacterium africanum | en |
dc.subject | Bacterial load | en |
dc.subject | Mycobacterium tuberculosis | en |
dc.subject | TB/MBLA | en |
dc.subject | QR Microbiology | en |
dc.subject | RC Internal medicine | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QR | en |
dc.subject.lcc | RC | en |
dc.title | Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Infection and Global Health Division | en |
dc.contributor.institution | University of St Andrews. Global Health Implementation Group | en |
dc.contributor.institution | University of St Andrews. Gillespie Group | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.identifier.doi | https://doi.org/10.3389/fmicb.2021.719531 | |
dc.description.status | Peer reviewed | en |
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