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dc.contributor.authorHulme, Charlotte H
dc.contributor.authorFuller, Heidi R
dc.contributor.authorRiddell, John
dc.contributor.authorShirran, Sally L
dc.contributor.authorBotting, Catherine H
dc.contributor.authorOsman, Aheed
dc.contributor.authorWright, Karina T
dc.date.accessioned2021-10-13T14:30:15Z
dc.date.available2021-10-13T14:30:15Z
dc.date.issued2021-10-02
dc.identifier.citationHulme , C H , Fuller , H R , Riddell , J , Shirran , S L , Botting , C H , Osman , A & Wright , K T 2021 , ' Investigation of the blood proteome in response to spinal cord injury in rodent models ' , Spinal cord , vol. First Online . https://doi.org/10.1038/s41393-021-00692-8en
dc.identifier.otherPURE: 276266220
dc.identifier.otherPURE UUID: 44ea8370-d112-4416-9529-c3ad96f2c4fe
dc.identifier.otherRIS: urn:90E65BECB0C2557DD91A102941021D66
dc.identifier.otherORCID: /0000-0003-3516-3507/work/101581458
dc.identifier.otherScopus: 85116347224
dc.identifier.urihttp://hdl.handle.net/10023/24134
dc.descriptionWe would like to thank the Institute of Orthopaedics and the Midlands Centre for Spinal Cord Injury (MCSI) for funding this research. This work was also supported by the Wellcome Trust [grant number 094476/Z/10/Z] which funded the purchase of the TripleTOF 5600 mass spectrometer at the BSRC Mass Spectrometry and Proteomics Facility, University of St Andrews.en
dc.description.abstractStudy Design: Explanatory and mechanistic study. Objective: A better understanding of the 'whole-body' response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying prognostic indicators for SCI. This study aimed to characterise the blood proteome following contusion or complete SCI compared to a sham injury in rat models. Setting: United Kingdom. Methods: Pooled blood samples from one and seven days after a contusion (serum; n = 5) or from 14 days and 112 days post-complete transection SCI (plasma; n = 8) and their sham-injured counterparts were subjected to independent iTRAQ nanoflow liquid chromatography tandem mass-spectrometry proteomic analyses. Pathway analyses of the proteins that were differentially abundant between SCI and their matched sham injured counterparts were completed to indicate biological pathways that may be changed in response to SCI. Results: Eleven and 42 proteins were differentially abundant (≥±2.0 FC; p ≤ 0.05) between the contusion SCI and sham injured animals at 24 h and seven days post-injury, respectively. Seven and tweleve proteins were differentially abundant between complete and sham injured rats at 14 and 112 days post-injury, respectively. Acute-phase response signalling and Liver X Receptor/Retinoic X Receptor activation were identified as differentially regulated pathways in both models of SCI. Conclusions: We have utilised longitudinal preclinical SCI models to provide an insight into the blood proteome changes that result following SCI and to highlight a number of biological pathways of interest for future studies.
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofSpinal corden
dc.rightsCopyright © Crown 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en
dc.subjectQH301 Biologyen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccRC0321en
dc.titleInvestigation of the blood proteome in response to spinal cord injury in rodent modelsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.identifier.doihttps://doi.org/10.1038/s41393-021-00692-8
dc.description.statusPeer revieweden


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