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dc.contributor.authorCoke, Mirela C
dc.contributor.authorMantelin, Sophie
dc.contributor.authorThorpe, Peter
dc.contributor.authorLilley, Catherine J
dc.contributor.authorWright, Kathryn M
dc.contributor.authorShaw, Daniel S
dc.contributor.authorChande, Adams
dc.contributor.authorJones, John T
dc.contributor.authorUrwin, Peter E
dc.identifier.citationCoke , M C , Mantelin , S , Thorpe , P , Lilley , C J , Wright , K M , Shaw , D S , Chande , A , Jones , J T & Urwin , P E 2021 , ' The GpIA7 effector from the potato cyst nematode Globodera pallida targets potato EBP1 and interferes with the plant cell cycle programme ' , Journal of Experimental Botany , vol. Advance Access , erab353 .
dc.identifier.otherBibtex: 10.1093/jxb/erab353
dc.descriptionThis work was funded through Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/F00334X/1 and BB/H000801/1. The James Hutton Institute receives funding from the Rural and Environment Science & Analytical Services Division of the Scottish Government.en
dc.description.abstractThe potato cyst nematode Globodera pallida acquires all of its nutrients from an elaborate feeding site that it establishes in a host plant root. Normal development of the root cells is re-programmed in a process coordinated by secreted nematode effector proteins. The biological function of the G. pallida GpIA7 effector was investigated in this study. GpIA7 is specifically expressed in the subventral pharyngeal glands of pre-parasitic stage nematodes. Ectopic expression of GpIA7 in potato plants affected plant growth and development, suggesting a potential role for this effector in feeding site establishment. Potato plants overexpressing GpIA7 were shorter, with reduced tuber weight and delayed flowering. We provide evidence that GpIA7 associates with the plant growth regulator StEBP1 (ErbB-3 epidermal growth factor receptor-binding protein 1).GpIA7 modulates the regulatory function of StEBP1, altering the expression level of downstream target genes, including ribonucleotide reductase 2, cyclin D3;1 and retinoblastoma related 1, which are downregulated in plants overexpressing GpIA7. We provide an insight into the molecular mechanism used by the nematode to manipulate the host cell cycle and provide evidence that this may rely, at least in part, on hindering the function of host EBP1.
dc.relation.ispartofJournal of Experimental Botanyen
dc.subjectCell cycleen
dc.subjectCyclin D3;1en
dc.subjectPotato RBR1en
dc.subjectQH426 Geneticsen
dc.subjectSB Plant cultureen
dc.titleThe GpIA7 effector from the potato cyst nematode Globodera pallida targets potato EBP1 and interferes with the plant cell cycle programmeen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.description.statusPeer revieweden

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