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dc.contributor.authorWallace, Megan A.
dc.contributor.authorCoffman, Kelsey A.
dc.contributor.authorGilbert, Clément
dc.contributor.authorRavindran, Sanjana
dc.contributor.authorAlbery, Gregory F.
dc.contributor.authorAbbott, Jessica
dc.contributor.authorArgyridou, Eliza
dc.contributor.authorBellosta, Paola
dc.contributor.authorBetancourt, Andrea J.
dc.contributor.authorColinet, Hervé
dc.contributor.authorEric, Katarina
dc.contributor.authorGlaser-Schmitt, Amanda
dc.contributor.authorGrath, Sonja
dc.contributor.authorJelic, Mihailo
dc.contributor.authorKankare, Maaria
dc.contributor.authorKozeretska, Iryna
dc.contributor.authorLoeschcke, Volker
dc.contributor.authorMontchamp-Moreau, Catherine
dc.contributor.authorOmetto, Lino
dc.contributor.authorOnder, Banu Sebnem
dc.contributor.authorOrengo, Dorcas J.
dc.contributor.authorParsch, John
dc.contributor.authorPascual, Marta
dc.contributor.authorPatenkovic, Aleksandra
dc.contributor.authorPuerma, Eva
dc.contributor.authorRitchie, Michael G.
dc.contributor.authorRota-Stabelli, Omar
dc.contributor.authorSchou, Mads Fristrup
dc.contributor.authorSerga, Svitlana V.
dc.contributor.authorStamenkovic-Radak, Marina
dc.contributor.authorTanaskovic, Marija
dc.contributor.authorVeselinovic, Marija Savic
dc.contributor.authorVieira, Jorge
dc.contributor.authorVieira, Cristina P.
dc.contributor.authorKapun, Martin
dc.contributor.authorFlatt, Thomas
dc.contributor.authorGonzález, Josefa
dc.contributor.authorStaubach, Fabian
dc.contributor.authorObbard, Darren J.
dc.date.accessioned2021-09-22T11:30:09Z
dc.date.available2021-09-22T11:30:09Z
dc.date.issued2021-04-01
dc.identifier.citationWallace , M A , Coffman , K A , Gilbert , C , Ravindran , S , Albery , G F , Abbott , J , Argyridou , E , Bellosta , P , Betancourt , A J , Colinet , H , Eric , K , Glaser-Schmitt , A , Grath , S , Jelic , M , Kankare , M , Kozeretska , I , Loeschcke , V , Montchamp-Moreau , C , Ometto , L , Onder , B S , Orengo , D J , Parsch , J , Pascual , M , Patenkovic , A , Puerma , E , Ritchie , M G , Rota-Stabelli , O , Schou , M F , Serga , S V , Stamenkovic-Radak , M , Tanaskovic , M , Veselinovic , M S , Vieira , J , Vieira , C P , Kapun , M , Flatt , T , González , J , Staubach , F & Obbard , D J 2021 , ' The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe ' , Virus Evolution , vol. 7 , no. 1 , veab031 . https://doi.org/10.1093/ve/veab031en
dc.identifier.issn2057-1577
dc.identifier.otherPURE: 275911775
dc.identifier.otherPURE UUID: c747331b-a15c-4a61-a077-07263eceb7ca
dc.identifier.otherScopus: 85114635292
dc.identifier.otherPubMedCentral: PMC8363768
dc.identifier.otherORCID: /0000-0001-7913-8675/work/100172449
dc.identifier.otherWOS: 000702030600028
dc.identifier.urihttps://hdl.handle.net/10023/23998
dc.descriptionFunding: M.W. was supported by the UK Natural Environmental Research Council through the E3 doctoral training programme (NE/L002558/1), and S.R. was supported by Wellcome Trust PhD programme (108905/Z/15/Z). A.B. received funding from BBSRC (grant number BB/P00685X/1). J.G. received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2014-CoG-647900) and from the Fundación Española para la Ciencia y la Tecnologia-Ministerio de Economía y Competitividad (FCT-15-10187). S.G. received funding from Deutsche Forschungsgemeinschaft (grant number GR 4495/2).en
dc.description.abstractDrosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here, we report fourteen DNA viruses detected in a metagenomic analysis of 6668 pool-sequenced Drosophila, sampled from forty-seven European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a double-stranded RNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to twelve segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2 per cent or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D.melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
dc.format.extent23
dc.language.isoeng
dc.relation.ispartofVirus Evolutionen
dc.rightsCopyright © The Author(s) 2021. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectAdintovirusen
dc.subjectBidnavirusen
dc.subjectDensovirusen
dc.subjectDNA virusen
dc.subjectDrosophilaen
dc.subjectEndogenous viral elementen
dc.subjectFilamentous virusen
dc.subjectGalbut virusen
dc.subjectNudivirusen
dc.subjectQR355 Virologyen
dc.subjectMicrobiologyen
dc.subjectVirologyen
dc.subjectDASen
dc.subjectACen
dc.subjectMCCen
dc.subject.lccQR355en
dc.titleThe discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europeen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biological Diversityen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.identifier.doihttps://doi.org/10.1093/ve/veab031
dc.description.statusPeer revieweden
dc.identifier.urlhttps://www.biorxiv.org/content/10.1101/2020.10.16.342956v2.article-infoen


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