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YAP translocation precedes cytoskeletal rearrangement in podocyte stress response : a podometric investigation of diabetic nephropathy
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dc.contributor.author | Haley, Kathryn | |
dc.contributor.author | Elshani, Mustafa | |
dc.contributor.author | Um, In Hwa | |
dc.contributor.author | Bell, Cameron | |
dc.contributor.author | Caie, Peter David | |
dc.contributor.author | Harrison, David James | |
dc.contributor.author | Reynolds, Paul Andrew | |
dc.date.accessioned | 2021-07-26T15:30:05Z | |
dc.date.available | 2021-07-26T15:30:05Z | |
dc.date.issued | 2021-07-15 | |
dc.identifier | 274298662 | |
dc.identifier | f98dcd98-1335-4439-b62e-bec9f9e96f0d | |
dc.identifier | 85111611999 | |
dc.identifier | 000679131200001 | |
dc.identifier.citation | Haley , K , Elshani , M , Um , I H , Bell , C , Caie , P D , Harrison , D J & Reynolds , P A 2021 , ' YAP translocation precedes cytoskeletal rearrangement in podocyte stress response : a podometric investigation of diabetic nephropathy ' , Frontiers in Physiology , vol. 12 , 625762 . https://doi.org/10.3389/fphys.2021.625762 | en |
dc.identifier.issn | 1664-042X | |
dc.identifier.other | ORCID: /0000-0001-8738-1245/work/97129695 | |
dc.identifier.other | ORCID: /0000-0001-9041-9988/work/97129830 | |
dc.identifier.other | ORCID: /0000-0002-0031-9850/work/97129928 | |
dc.identifier.other | ORCID: /0000-0001-9999-4292/work/158122929 | |
dc.identifier.uri | https://hdl.handle.net/10023/23637 | |
dc.description | KH was funded by a University of St Andrews 600th Anniversary Ph.D. scholarship. ME and DH were supported by NHS Lothian. | en |
dc.description.abstract | Podocyte loss plays a pivotal role in the pathogenesis of glomerular disease. However, the mechanisms underlying podocyte damage and loss remain poorly understood. Although detachment of viable cells has been documented in experimental Diabetic Nephropathy, correlations between reduced podocyte density and disease severity have not yet been established. YAP, a mechanosensing protein, has recently been shown to correlate with glomerular disease progression, however, the underlying mechanism has yet to be fully elucidated. In this study, we sought to document podocyte density in Diabetic Nephropathy using an amended podometric methodology, and to investigate the interplay between YAP and cytoskeletal integrity during podocyte injury. Podocyte density was quantified using TLE4 and GLEPP1 multiplexed immunofluorescence. Fourteen Diabetic Nephropathy cases were analyzed for both podocyte density and cytoplasmic translocation of YAP via automated image analysis. We demonstrate a significant decrease in podocyte density in Grade III/IV cases (124.5 per 106 μm3) relative to Grade I/II cases (226 per 106 μm3) (Student’s t-test, p<0.001), and further show that YAP translocation precedes cytoskeletal rearrangement following injury. Based on these findings we hypothesize that a significant decrease in podocyte density in late grade Diabetic Nephropathy may be explained by early cytoplasmic translocation of YAP. | |
dc.format.extent | 10 | |
dc.format.extent | 6752683 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Physiology | en |
dc.subject | Podocytes | en |
dc.subject | Diabetic nephropathy | en |
dc.subject | Podometrics | en |
dc.subject | YAP | en |
dc.subject | Automated image analysis | en |
dc.subject | Hippo signaling | en |
dc.subject | QH426 Genetics | en |
dc.subject | 3rd-DAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH426 | en |
dc.title | YAP translocation precedes cytoskeletal rearrangement in podocyte stress response : a podometric investigation of diabetic nephropathy | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.identifier.doi | 10.3389/fphys.2021.625762 | |
dc.description.status | Peer reviewed | en |
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