Files in this item
Real-time imaging of cellular forces using optical interference
Item metadata
dc.contributor.author | Meek, Andrew Thomas | |
dc.contributor.author | Kronenberg, Nils Michael | |
dc.contributor.author | Morton, Andrew | |
dc.contributor.author | Liehm, Philipp | |
dc.contributor.author | Murawski, Jan | |
dc.contributor.author | Dalaka, Eleni | |
dc.contributor.author | Booth, Jonathan Hunter | |
dc.contributor.author | Powis, Simon John | |
dc.contributor.author | Gather, Malte Christian | |
dc.date.accessioned | 2021-06-15T12:30:06Z | |
dc.date.available | 2021-06-15T12:30:06Z | |
dc.date.issued | 2021-06-11 | |
dc.identifier.citation | Meek , A T , Kronenberg , N M , Morton , A , Liehm , P , Murawski , J , Dalaka , E , Booth , J H , Powis , S J & Gather , M C 2021 , ' Real-time imaging of cellular forces using optical interference ' , Nature Communications , vol. 12 , 3552 . https://doi.org/10.1038/s41467-021-23734-4 | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | PURE: 273902502 | |
dc.identifier.other | PURE UUID: 5e7dbb60-20c1-49c2-b3e2-da7ee3381c9a | |
dc.identifier.other | ORCID: /0000-0003-4218-2984/work/95772297 | |
dc.identifier.other | ORCID: /0000-0002-4857-5562/work/95772548 | |
dc.identifier.other | ORCID: /0000-0003-3306-7466/work/95772720 | |
dc.identifier.other | WOS: 000663756500001 | |
dc.identifier.other | Scopus: 85107739075 | |
dc.identifier.uri | https://hdl.handle.net/10023/23363 | |
dc.description | Funding: This research was financially supported by an EPSRC Programme Grant (EP/P030017/1), by the European Research Council under the European Union’s Horizon 2020 Framework Programme (ERC StG ABLASE, 640012), and by the EPSRC Doctoral Training Partnership (EP/M508214/1, EP/L505079/1). M.C.G. acknowledges funding from the Alexander von Humboldt Stiftung (Humboldt-Professorship). | en |
dc.description.abstract | Important dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution. | |
dc.format.extent | 11 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Communications | en |
dc.rights | Copyright © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
dc.subject | Cell adhesion | en |
dc.subject | Cellular imagining | en |
dc.subject | IMagining techniques | en |
dc.subject | Interference microscopy | en |
dc.subject | QH301 Biology | en |
dc.subject | QC Physics | en |
dc.subject | T Technology | en |
dc.subject | DAS | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | T | en |
dc.title | Real-time imaging of cellular forces using optical interference | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | European Research Council | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. St Andrews Bioinformatics Unit | en |
dc.identifier.doi | https://doi.org/10.1038/s41467-021-23734-4 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | 640012 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.