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dc.contributor.authorJones, Chloe E.
dc.contributor.authorTan, Wenfang Spring
dc.contributor.authorGrey, Finn
dc.contributor.authorHughes, David John
dc.date.accessioned2021-05-31T09:30:02Z
dc.date.available2021-05-31T09:30:02Z
dc.date.issued2021-05-21
dc.identifier.citationJones , C E , Tan , W S , Grey , F & Hughes , D J 2021 , ' Discovering antiviral restriction factors and pathways using genetic screens ' , Journal of General Virology , vol. 102 , no. 5 , 0001603 . https://doi.org/10.1099/jgv.0.001603en
dc.identifier.issn0022-1317
dc.identifier.otherPURE: 274387465
dc.identifier.otherPURE UUID: dd1a426b-08ff-4a0c-b715-482619cc01d1
dc.identifier.otherPubMed: 34020727
dc.identifier.otherScopus: 85106737507
dc.identifier.otherWOS: 000659946800008
dc.identifier.urihttp://hdl.handle.net/10023/23279
dc.descriptionResearch in the Hughes lab is supported by a grant from the Academy of Medical Sciences (SFB003/1028), a grant from Tenovus Scotland (T20/63), and The Wellcome Trust Institutional Strategic Support Fund (ISSF). Research in the Gray lab is supported Medical Research Council (MR/N001796/1) and the Biotechnology and Biological Sciences Research Council (BBS/E/D/20002172). C. E. J. is supported by a University of St Andrews Ph.D. scholarship.en
dc.description.abstractViral infections activate the powerful interferon (IFN) response that induces the expression of several hundred IFN stimulated genes (ISGs). The principal role of this extensive response is to create an unfavourable environment for virus replication and to limit spread; however, untangling the biological consequences of this large response is complicated. In addition to a seemingly high degree of redundancy, several ISGs are usually required in combination to limit infection as individual ISGs often have low to moderate antiviral activity. Furthermore, what ISG or combination of ISGs are antiviral for a given virus is usually not known. For these reasons, and that the function(s) of many ISGs remains unexplored, genome-wide approaches are well placed to investigate what aspects of this response results in an appropriate, virus-specific phenotype. This review discusses the advances screening approaches have provided for the study of host defence mechanisms, including CRISPR/Cas9, ISG expression libraries and RNAi technologies.
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofJournal of General Virologyen
dc.rightsCopyright © 2021 The Authors. This is an Open Access article published by the Microbiology Society under the Creative Commons Attribution License.en
dc.subjectInnate immunityen
dc.subjectCRISPR–Cas9en
dc.subjectGenome-wide screensen
dc.subjectRNAien
dc.subjectAntiviral immunityen
dc.subjectInterferonen
dc.subjectQR355 Virologyen
dc.subject.lccQR355en
dc.titleDiscovering antiviral restriction factors and pathways using genetic screensen
dc.typeJournal itemen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1099/jgv.0.001603
dc.description.statusPeer revieweden


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