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dc.contributor.authorDa Rocha, Martine
dc.contributor.authorBournaud, Caroline
dc.contributor.authorDazenière, Julie
dc.contributor.authorThorpe, Peter
dc.contributor.authorBailly-Bechet, Marc
dc.contributor.authorPellegrin, Clément
dc.contributor.authorPéré, Arthur
dc.contributor.authorGrynberg, Priscila
dc.contributor.authorPerfus-Barbeoch, Laetitia
dc.contributor.authorEves-van den Akker, Sebastian
dc.contributor.authorDanchin, Etienne G. J.
dc.date.accessioned2021-05-24T14:30:15Z
dc.date.available2021-05-24T14:30:15Z
dc.date.issued2021-05-18
dc.identifier.citationDa Rocha , M , Bournaud , C , Dazenière , J , Thorpe , P , Bailly-Bechet , M , Pellegrin , C , Péré , A , Grynberg , P , Perfus-Barbeoch , L , Eves-van den Akker , S & Danchin , E G J 2021 , ' Genome expression dynamics reveal the parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genes ' , Genes , vol. 12 , no. 5 , e771 . https://doi.org/10.3390/genes12050771en
dc.identifier.issn2073-4425
dc.identifier.otherPURE: 274343883
dc.identifier.otherPURE UUID: 9ae40ed0-2278-4ecc-9cae-5d41793b59f8
dc.identifier.otherJisc: 9b020fe1d8ee4601b4ef429a2d27ea7d
dc.identifier.otherScopus: 85107386457
dc.identifier.otherWOS: 000654012500001
dc.identifier.urihttps://hdl.handle.net/10023/23239
dc.descriptionWork on plant-parasitic nematodes at the University of Cambridge is supported by DEFRA license 125034/359149/3, and funded by BBSRC grants BB/R011311/1, BB/N021908/1, and BB/S006397/1. C.P and C.B are supported by Marie Skłodowska-Curie Actions Individual Fellowships.en
dc.description.abstractRoot-knot nematodes (genus Meloidogyne) are the major contributor to crop losses caused by nematodes. These nematodes secrete effector proteins into the plant, derived from two sets of pharyngeal gland cells, to manipulate host physiology and immunity. Successful completion of the life cycle, involving successive molts from egg to adult, covers morphologically and functionally distinct stages and will require precise control of gene expression, including effector genes. The details of how root-knot nematodes regulate transcription remain sparse. Here, we report a life stage-specific transcriptome of Meloidogyne incognita. Combined with an available annotated genome, we explore the spatio-temporal regulation of gene expression. We reveal gene expression clusters and predicted functions that accompany the major developmental transitions. Focusing on effectors, we identify a putative cis-regulatory motif associated with expression in the dorsal glands, providing an insight into effector regulation. We combine the presence of this motif with several other criteria to predict a novel set of putative dorsal gland effectors. Finally, we show this motif, and thereby its utility, is broadly conserved across the Meloidogyne genus, and we name it Mel-DOG. Taken together, we provide the first genome-wide analysis of spatio-temporal gene expression in a root-knot nematode and identify a new set of candidate effector genes that will guide future functional analyses. 
dc.format.extent25
dc.language.isoeng
dc.relation.ispartofGenesen
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/).en
dc.subjectGenomeen
dc.subjectTranscriptomeen
dc.subjectPhytoparasitismen
dc.subjectGene expression regulationen
dc.subjectMeloidogyneen
dc.subjectNematodeen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectSB Plant cultureen
dc.subject3rd-DASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.subject.lccSBen
dc.titleGenome expression dynamics reveal the parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.identifier.doihttps://doi.org/10.3390/genes12050771
dc.description.statusPeer revieweden


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