Show simple item record

Files in this item


Item metadata

dc.contributor.authorThanh Hai, Hoang
dc.contributor.authorSabiiti, Wilber
dc.contributor.authorAnh Thu, Do Dang
dc.contributor.authorHoan Phu, Nguyen
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorThwaites, Guy
dc.contributor.authorThuy Thuong Thuong, Nguyen
dc.identifier.citationThanh Hai , H , Sabiiti , W , Anh Thu , D D , Hoan Phu , N , Gillespie , S H , Thwaites , G & Thuy Thuong Thuong , N 2021 , ' Evaluation of the molecular bacterial load assay for detecting viable Mycobacterium tuberculosis in cerebrospinal fluid before and during tuberculous meningitis treatment ' , Tuberculosis , vol. 128 , 102084 .
dc.identifier.otherORCID: /0000-0001-6537-7712/work/93514676
dc.identifier.otherORCID: /0000-0002-4742-2791/work/93515193
dc.descriptionFunding: This work was supported by the Wellcome Trust (206724/Z/17/Z to NTTT and 106680/B/14/Z to GT).en
dc.description.abstractNew tools to monitor treatment response and predict outcome from tuberculous meningitis (TBM) are urgently required. We retrospectively evaluated the 16S rRNA-based molecular bacterial load assay (MBLA) to quantify viable Mycobacterium tuberculosis in serial cerebrospinal fluid (CSF) from adults with TBM. 187 CSF samples were collected before and during the first two months of treatment from 99 adults TBM, comprising 56 definite, 43 probable or possible TBM, and 18 non-TBM and preserved at −80 °C prior to MBLA. We compared MBLA against MGIT culture, GeneXpert MTB/RIF (Xpert) and Ziehl-Neelsen (ZN) smear. Before treatment, MBLA was positive in 34/99 (34.3%), significantly lower than MGIT 47/99 (47.5%), Xpert 51/99 (51.5%) and ZN smear 55/99 (55.5%). After one month of treatment, MBLA and MGIT were positive in 3/38 (7.9%) and 4/38 (10.5%), respectively, whereas Xpert and ZN smear remained positive in 19/38 (50.0%) and 18/38 (47.4%). In summary, MBLA was less likely to detect CSF bacteria before the start of treatment compared with MGIT culture, Xpert and ZN smear. MBLA and MGIT positivity fell during treatment because of detecting only viable bacteria, whereas Xpert and ZN smear remained positive for longer because of detecting both live and dead bacteria. Sample storage and processing may have reduced MBLA-detectable viable bacteria; and sampling earlier in treatment may yield more useful results. Prospective studies with CSF sampling after 1–2 weeks are warranted.
dc.subjectTuberculosis meningitisen
dc.subjectCerebrospinal fluiden
dc.subjectMcobacterium tuberculoisen
dc.subjectViable bacterial loaden
dc.subject16S rRNAen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleEvaluation of the molecular bacterial load assay for detecting viable Mycobacterium tuberculosis in cerebrospinal fluid before and during tuberculous meningitis treatmenten
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record