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DEER and RIDME measurements of the nitroxide-spin labelled copper-bound amine oxidase homodimer from arthrobacter globiformis

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Russell_2021_AMR_DEER_CC.pdf (945.0Kb)
Date
29/03/2021
Author
Russell, Hannah
Stewart, Rachel
Prior, Christopher
Oganesyan, Vasily S.
Gaule, Thembaninkosi G.
Lovett, Janet E.
Keywords
QC Physics
DAS
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Abstract
In the study of biological structures, pulse dipolar spectroscopy (PDS) is used to elucidate spin–spin distances at nanometre-scale by measuring dipole–dipole interactions between paramagnetic centres. The PDS methods of Double Electron Electron Resonance (DEER) and Relaxation Induced Dipolar Modulation Enhancement (RIDME) are employed, and their results compared, for the measurement of the dipolar coupling between nitroxide spin labels and copper-II (Cu(II)) paramagnetic centres within the copper amine oxidase from Arthrobacter globiformis (AGAO). The distance distribution results obtained indicate that two distinct distances can be measured, with the longer of these at c.a. 5 nm. Conditions for optimising the RIDME experiment such that it may outperform DEER for these long distances are discussed. Modelling methods are used to show that the distances obtained after data analysis are consistent with the structure of AGAO.
Citation
Russell , H , Stewart , R , Prior , C , Oganesyan , V S , Gaule , T G & Lovett , J E 2021 , ' DEER and RIDME measurements of the nitroxide-spin labelled copper-bound amine oxidase homodimer from arthrobacter globiformis ' , Applied Magnetic Resonance , vol. First Online . https://doi.org/10.1007/s00723-021-01321-6
Publication
Applied Magnetic Resonance
Status
Peer reviewed
DOI
https://doi.org/10.1007/s00723-021-01321-6
ISSN
0937-9347
Type
Journal article
Rights
Copyright © The Author(s) 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Description
Authors acknowledge financial support from EPSRC (studentship to HR EP/R513337/1, Grant support to VSO EP/P007554/1, Grant support to TGG EP/L015005/1), The Royal Society (URF to JEL URF150698), and The Wellcome Trust for a multi-user equipment grant (099149/Z/12/Z).
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  • University of St Andrews Research
URL
https://link.springer.com/article/10.1007%2Fs00723-021-01321-6#Sec19
URI
http://hdl.handle.net/10023/22985

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