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Innate sensing of HIV-infected cells
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dc.contributor.author | Lepelley, Alice | |
dc.contributor.author | Louis, Stephanie | |
dc.contributor.author | Sourisseau, Marion | |
dc.contributor.author | Law, Helen K. W. | |
dc.contributor.author | Pothlichet, Julien | |
dc.contributor.author | Schilte, Clementine | |
dc.contributor.author | Chaperot, Laurence | |
dc.contributor.author | Plumas, Joel | |
dc.contributor.author | Randall, Richard Edward | |
dc.contributor.author | Si-Tahar, Mustapha | |
dc.contributor.author | Mammano, Fabrizio | |
dc.contributor.author | Albert, Matthew L. | |
dc.contributor.author | Schwartz, Olivier | |
dc.date.accessioned | 2012-01-20T10:31:10Z | |
dc.date.available | 2012-01-20T10:31:10Z | |
dc.date.issued | 2011-02 | |
dc.identifier | 16587272 | |
dc.identifier | 8ecad09d-cb90-40cf-aecb-1ab28a496618 | |
dc.identifier | 000287698200022 | |
dc.identifier | 79952217825 | |
dc.identifier.citation | Lepelley , A , Louis , S , Sourisseau , M , Law , H K W , Pothlichet , J , Schilte , C , Chaperot , L , Plumas , J , Randall , R E , Si-Tahar , M , Mammano , F , Albert , M L & Schwartz , O 2011 , ' Innate sensing of HIV-infected cells ' , PLoS Pathogens , vol. 7 , no. 2 , e1001284 . https://doi.org/10.1371/journal.ppat.1001284 | en |
dc.identifier.issn | 1553-7366 | |
dc.identifier.other | ORCID: /0000-0002-9304-6678/work/60427008 | |
dc.identifier.uri | https://hdl.handle.net/10023/2178 | |
dc.description.abstract | Cell-free HIV-1 virions are poor stimulators of type I interferon (IFN) production. We examined here how HIV-infected cells are recognized by plasmacytoid dendritic cells (pDCs) and by other cells. We show that infected lymphocytes are more potent inducers of IFN than virions. There are target cell-type differences in the recognition of infected lymphocytes. In primary pDCs and pDC-like cells, recognition occurs in large part through TLR7, as demonstrated by the use of inhibitors and by TLR7 silencing. Donor cells expressing replication-defective viruses, carrying mutated reverse transcriptase, integrase or nucleocapsid proteins induced IFN production by target cells as potently as wild-type virus. In contrast, Env-deleted or fusion defective HIV-1 mutants were less efficient, suggesting that in addition to TLR7, cytoplasmic cellular sensors may also mediate sensing of infected cells. Furthermore, in a model of TLR7-negative cells, we demonstrate that the IRF3 pathway, through a process requiring access of incoming viral material to the cytoplasm, allows sensing of HIV-infected lymphocytes. Therefore, detection of HIV-infected lymphocytes occurs through both endosomal and cytoplasmic pathways. Characterization of the mechanisms of innate recognition of HIV-infected cells allows a better understanding of the pathogenic and exacerbated immunologic events associated with HIV infection. | |
dc.format.extent | 15 | |
dc.format.extent | 2112920 | |
dc.language.iso | eng | |
dc.relation.ispartof | PLoS Pathogens | en |
dc.subject | Human-immunodeficiency-virus | en |
dc.subject | Plasmacytoid dendritic cells | en |
dc.subject | CD4(+) T-cells | en |
dc.subject | I interferon-production | en |
dc.subject | RIG-I | en |
dc.subject | Antiviral responses | en |
dc.subject | Alpha-interferon | en |
dc.subject | IFN-alpha | en |
dc.subject | Viral-infection | en |
dc.subject | Adapter protein | en |
dc.subject | QR355 Virology | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QR355 | en |
dc.title | Innate sensing of HIV-infected cells | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1371/journal.ppat.1001284 | |
dc.description.status | Peer reviewed | en |
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