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dc.contributor.authorKranse, Olaf
dc.contributor.authorBeasley, Helen
dc.contributor.authorAdams, Sally
dc.contributor.authorPires-daSilva, Andre
dc.contributor.authorBell, Christopher
dc.contributor.authorLilley, Catherine J
dc.contributor.authorUrwin, Peter E
dc.contributor.authorBird, David
dc.contributor.authorMiska, Eric
dc.contributor.authorSmant, Geert
dc.contributor.authorGheysen, Godelieve
dc.contributor.authorJones, John
dc.contributor.authorViney, Mark
dc.contributor.authorAbad, Pierre
dc.contributor.authorMaier, Thomas R
dc.contributor.authorBaum, Thomas J
dc.contributor.authorSiddique, Shahid
dc.contributor.authorWilliamson, Valerie
dc.contributor.authorAkay, Alper
dc.contributor.authorEves-van den Akker, Sebastian
dc.date.accessioned2021-03-12T17:30:14Z
dc.date.available2021-03-12T17:30:14Z
dc.date.issued2021-02
dc.identifier.citationKranse , O , Beasley , H , Adams , S , Pires-daSilva , A , Bell , C , Lilley , C J , Urwin , P E , Bird , D , Miska , E , Smant , G , Gheysen , G , Jones , J , Viney , M , Abad , P , Maier , T R , Baum , T J , Siddique , S , Williamson , V , Akay , A & Eves-van den Akker , S 2021 , ' Toward genetic modification of plant-parasitic nematodes : delivery of macromolecules to adults and expression of exogenous mRNA in second stage juveniles ' , G3: Genes, Genomes, Genetics , vol. 11 , no. 2 , jkaa058 . https://doi.org/10.1093/g3journal/jkaa058en
dc.identifier.issn2160-1836
dc.identifier.otherPURE: 273294888
dc.identifier.otherPURE UUID: 585a0128-821c-4dc2-9934-ed9515476acf
dc.identifier.otherJisc: b6e939d0704f4d51bfc91b088d49b913
dc.identifier.otherScopus: 85101489915
dc.identifier.otherWOS: 000651850600020
dc.identifier.urihttp://hdl.handle.net/10023/21629
dc.descriptionFunding: Work on plant-parasitic nematodes at the University of Cambridge is supported by DEFRA licence 125034/359149/3, and was funded by Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/R011311/1, BB/N021908/1, and BB/S006397/1, a Synthego Genome Engineer grant, and a Genewiz grant. A.A. was supported by a Wellcome/Newton trust Institutional Strategic Support Fund grant and a UKRI Future Leaders Fellowship MR/S033769/1. C.J.L. was supported by BBSRC grant BB/N016866/1. J.J. receives funding from the Scottish Government Rural and Environmental Science and Analytical Services division. T.R.M. and T.J.B. were supported by grants from the Iowa Soybean Association and by State of Iowa and Hatch funds. A.P.S. and S.A. were supported by grants RPG-2016-089 and BB/L019884/1.en
dc.description.abstractPlant-parasitic nematodes are a continuing threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics: forward genetics is largely restricted to studies of natural variation in populations and reverse genetics is entirely reliant on RNA interference. There is an expectation that the development of functional genetic tools would accelerate the progress of research on plant-parasitic nematodes, and hence the development of novel control solutions. Here, we develop some of the foundational biology required to deliver a functional genetic tool kit in plant-parasitic nematodes. We characterize the gonads of male Heterodera schachtii and Meloidogyne hapla in the context of spermatogenesis. We test and optimize various methods for the delivery, expression, and/or detection of exogenous nucleic acids in plant-parasitic nematodes. We demonstrate that delivery of macromolecules to cyst and root knot nematode male germlines is difficult, but possible. Similarly, we demonstrate the delivery of oligonucleotides to root knot nematode gametes. Finally, we develop a transient expression system in plant-parasitic nematodes by demonstrating the delivery and expression of exogenous mRNA encoding various reporter genes throughout the body of H. schachtii juveniles using lipofectamine-based transfection. We anticipate these developments to be independently useful, will expedite the development of genetic modification tools for plant-parasitic nematodes, and ultimately catalyze research on a group of nematodes that threaten global food security.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofG3: Genes, Genomes, Geneticsen
dc.rightsCopyright © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectPlant-parasitic nematodesen
dc.subjectTransient expressionen
dc.subjectGenetic modificationen
dc.subjectLipofectionen
dc.subjectTransformationen
dc.subjectGermlineen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectS Agricultureen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.subject.lccSen
dc.titleToward genetic modification of plant-parasitic nematodes : delivery of macromolecules to adults and expression of exogenous mRNA in second stage juvenilesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.Arctic Research Centreen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.St Andrews Bioinformatics Uniten
dc.identifier.doihttps://doi.org/10.1093/g3journal/jkaa058
dc.description.statusPeer revieweden


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