Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorMarkakis, Konstantinos
dc.contributor.authorLowe, Phillip T.
dc.contributor.authorDavison-Gates, Liam
dc.contributor.authorO'Hagan, David
dc.contributor.authorRosser, Susan J.
dc.contributor.authorElfick, Alistair
dc.date.accessioned2021-03-03T00:35:57Z
dc.date.available2021-03-03T00:35:57Z
dc.date.issued2020-03-03
dc.identifier.citationMarkakis , K , Lowe , P T , Davison-Gates , L , O'Hagan , D , Rosser , S J & Elfick , A 2020 , ' An engineered E. coli strain for direct in vivo fluorination ' , ChemBioChem , vol. Early View . https://doi.org/10.1002/cbic.202000051en
dc.identifier.issn1439-4227
dc.identifier.otherPURE: 266931333
dc.identifier.otherPURE UUID: 293582e0-90a9-4365-9d4f-b421ca73ace1
dc.identifier.otherRIS: urn:EDF0DD46A940C937344D0F6ACB17F17F
dc.identifier.otherORCID: /0000-0002-0510-5552/work/70919794
dc.identifier.otherScopus: 85081569900
dc.identifier.otherWOS: 000517647500001
dc.identifier.urihttp://hdl.handle.net/10023/21543
dc.descriptionThis work was funded by the Industrial Biotechnology Innovation Centre (IBioIC) with support from GlaxoSmithKline, and also the EU Horizon 2020 (Sinfonia consortia).en
dc.description.abstractSelectively fluorinated compounds are found frequently in pharmaceutical and agrochemical products where currently 25–30 % of optimised compounds emerge from development containing at least one fluorine atom. There are many methods for the site‐specific introduction of fluorine, but all are chemical and they often use environmentally challenging reagents. Biochemical processes for C−F bond formation are attractive, but they are extremely rare. In this work, the fluorinase enzyme, originally identified from the actinomycete bacterium Streptomyces cattleya, is engineered into Escherichia coli in such a manner that the organism is able to produce 5′‐fluorodeoxyadenosine (5′‐FDA) from S‐adenosyl‐l‐methionine (SAM) and fluoride in live E. coli cells. Success required the introduction of a SAM transporter and deletion of the endogenous fluoride efflux capacity in order to generate an E. coli host that has the potential for future engineering of more elaborate fluorometabolites.
dc.language.isoeng
dc.relation.ispartofChemBioChemen
dc.rightsCopyright © 2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1002/cbic.202000051en
dc.subjectEscherichia colien
dc.subjectFluoride channelsen
dc.subjectFluorinasesen
dc.subjectHalogenationsen
dc.subjectSAM transportersen
dc.subjectQD Chemistryen
dc.subjectDASen
dc.subject.lccQDen
dc.titleAn engineered E. coli strain for direct in vivo fluorinationen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.identifier.doihttps://doi.org/10.1002/cbic.202000051
dc.description.statusPeer revieweden
dc.date.embargoedUntil2021-03-03


This item appears in the following Collection(s)

Show simple item record