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dc.contributor.authorRichmond, Gregory S.
dc.contributor.authorGibellini, Federica
dc.contributor.authorYoung, Simon A.
dc.contributor.authorMajor, Louise
dc.contributor.authorDenton, Helen
dc.contributor.authorLilley, Alison
dc.contributor.authorSmith, Terry K
dc.identifier.citationRichmond , G S , Gibellini , F , Young , S A , Major , L , Denton , H , Lilley , A & Smith , T K 2010 , ' Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei ' Parasitology , vol. 137 , no. 9 , pp. 1357-1392 .
dc.identifier.otherPURE: 3438721
dc.identifier.otherPURE UUID: 339f9e08-2192-4a27-a12c-1f26aa196f55
dc.identifier.otherWOS: 000280418400008
dc.identifier.otherScopus: 77953489045
dc.identifier.otherORCID: /0000-0001-5287-4488/work/51010264
dc.identifier.otherORCID: /0000-0003-1072-905X/work/38796295
dc.description.abstractThe biological membranes of Trypanosonza brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of similar to 500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.
dc.rights(c)2010 Cambridge University Pressen
dc.subjectTrypanosonza brucei mass spectrometryen
dc.subjectGpi-anchored proteinsen
dc.subjectDe-novo synthesisen
dc.subjectKennedy pathwayen
dc.subjectSphingolipid synthesisven
dc.subjectAfrican trypanosomesen
dc.subjectMyristate exchangeen
dc.subjectCulture formsen
dc.subjectQH301 Biologyen
dc.subjectQD Chemistryen
dc.titleLipidomic analysis of bloodstream and procyclic form Trypanosoma bruceien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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