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dc.contributor.authorVarypatakis, Kyriakos
dc.contributor.authorVéronneau, Pierre-Yves
dc.contributor.authorThorpe, Peter
dc.contributor.authorCock, Peter J A
dc.contributor.authorTze- Yin Lim, Joanne
dc.contributor.authorArmstrong, Miles R.
dc.contributor.authorJanakowski, Sławomir
dc.contributor.authorSobczak, Mirosław
dc.contributor.authorHein, Ingo
dc.contributor.authorMimee, Benjamin
dc.contributor.authorJones, John
dc.contributor.authorBlok, Vivian
dc.date.accessioned2020-12-07T15:58:59Z
dc.date.available2020-12-07T15:58:59Z
dc.date.issued2020-11-28
dc.identifier.citationVarypatakis , K , Véronneau , P-Y , Thorpe , P , Cock , P J A , Tze- Yin Lim , J , Armstrong , M R , Janakowski , S , Sobczak , M , Hein , I , Mimee , B , Jones , J & Blok , V 2020 , ' The genomic impact of selection for virulence against resistance in the potato cyst nematode, Globodera pallida ' , Genes , vol. 11 , no. 12 , 1429 . https://doi.org/10.3390/genes11121429en
dc.identifier.issn2073-4425
dc.identifier.otherPURE: 271433916
dc.identifier.otherPURE UUID: ad6cc4eb-238e-460d-b782-3a0ba1c41195
dc.identifier.otherScopus: 85097036794
dc.identifier.otherWOS: 000602052100001
dc.identifier.urihttp://hdl.handle.net/10023/21108
dc.descriptionThis work was funded through an AHDB PhD award, the USDA GLOBAL Project and by the Rural and Environmental Science and Analytical Services division of the Scottish Government. Bioinformatics and computational analyses for the generation of the new G. pallida genome assembly were supported by the University of St Andrews Bioinformatics Unit, which is funded by a Wellcome Trust ISSF award (grant 105621/Z/14/Z).en
dc.description.abstractAlthough the use of natural resistance is the most effective management approach against the potato cyst nematode (PCN) Globodera pallida, the existence of pathotypes with different virulence characteristics constitutes a constraint towards this goal. Two resistance sources, GpaV (from Solanum vernei) and H3 from S. tuberosum ssp. andigena CPC2802 (from the Commonwealth Potato Collection) are widely used in potato breeding programmes in European potato industry. However, the use of resistant cultivars may drive strong selection towards virulence, which allows the increase in frequency of virulent alleles in the population and therefore, the emergence of highly virulent nematode lineages. This study aimed to identify Avirulence (Avr) genes in G. pallida populations selected for virulence on the above resistance sources, and the genomic impact of selection processes on the nematode. The selection drive in the populations was found to be specific to their genetic background. At the genomic level, 11 genes were found that represent candidate Avr genes. Most of the variant calls determining selection were associated with H3-selected populations, while many of them seem to be organised in genomic islands facilitating selection evolution. These phenotypic and genomic findings combined with histological studies performed revealed potential mechanisms underlying selection in G. pallida.
dc.format.extent21
dc.language.isoeng
dc.relation.ispartofGenesen
dc.rightsCopyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectPotato cyst nematodesen
dc.subjectGlobodera pallidaen
dc.subjectNext generation sequencingen
dc.subjectTarget enrichment sequencingen
dc.subjectWhole genome sequencingen
dc.subjectSelectionen
dc.subjectVirulenceen
dc.subjectEffectorsen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectSB Plant cultureen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.subject.lccSBen
dc.titleThe genomic impact of selection for virulence against resistance in the potato cyst nematode, Globodera pallidaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.3390/genes11121429
dc.description.statusPeer revieweden


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