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Comparison of ePlex respiratory pathogen panel with laboratory-developed real-time PCR assays for detection of respiratory pathogens
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dc.contributor.author | Nijhuis, R. H.T. | |
dc.contributor.author | Guerendiain, D. | |
dc.contributor.author | Claas, E. C.J. | |
dc.contributor.author | Templeton, K. E. | |
dc.date.accessioned | 2020-11-23T15:30:42Z | |
dc.date.available | 2020-11-23T15:30:42Z | |
dc.date.issued | 2017-06 | |
dc.identifier.citation | Nijhuis , R H T , Guerendiain , D , Claas , E C J & Templeton , K E 2017 , ' Comparison of ePlex respiratory pathogen panel with laboratory-developed real-time PCR assays for detection of respiratory pathogens ' , Journal of Clinical Microbiology , vol. 55 , no. 6 , pp. 1938-1945 . https://doi.org/10.1128/JCM.00221-17 | en |
dc.identifier.issn | 0095-1137 | |
dc.identifier.other | PURE: 270078460 | |
dc.identifier.other | PURE UUID: f2f7f981-c87e-4a1d-9f8c-1a43e1020115 | |
dc.identifier.other | Scopus: 85019572081 | |
dc.identifier.other | PubMed: 28404682 | |
dc.identifier.other | ORCID: /0000-0002-7536-1308/work/80257961 | |
dc.identifier.uri | http://hdl.handle.net/10023/21048 | |
dc.description.abstract | Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex respiratory pathogen panel (RP panel) is a novel molecular biology-based assay, developed by GenMark Diagnostics, Inc. (Carlsbad, CA), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens. A total of 343 clinical specimens as well as 29 external quality assessment (EQA) specimens and 2 different Middle East respiratory syndrome coronavirus isolates have been assessed in this study. The RP panel showed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the clinical specimens. All pathogens present in clinical samples and EQA samples with a threshold cycle (CT) value of <30 were detected correctly using the RP panel. The RP panel detected 17 additional pathogens, 7 of which could be confirmed by discrepant testing. In conclusion, this study shows excellent performance of the RP panel in comparison to real-time PCR assays for the detection of respiratory pathogens. The ePlex system provided a large amount of useful diagnostic data within a short time frame, with minimal hands-on time, and can therefore potentially be used for rapid diagnostic sample-to-answer testing, in either a laboratory or a decentralized setting. | |
dc.format.extent | 8 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Clinical Microbiology | en |
dc.rights | Copyright © 2017 Nijhuis et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International license. | en |
dc.subject | Influenza | en |
dc.subject | Point-of-care testing | en |
dc.subject | Rapid diagnostics | en |
dc.subject | Respiratory pathogens | en |
dc.subject | Sample-to-answer | en |
dc.subject | QR Microbiology | en |
dc.subject | RB Pathology | en |
dc.subject | Microbiology (medical) | en |
dc.subject | DAS | en |
dc.subject.lcc | QR | en |
dc.subject.lcc | RB | en |
dc.title | Comparison of ePlex respiratory pathogen panel with laboratory-developed real-time PCR assays for detection of respiratory pathogens | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Infection and Global Health Division | en |
dc.identifier.doi | https://doi.org/10.1128/JCM.00221-17 | |
dc.description.status | Peer reviewed | en |
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