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dc.contributor.authorBoers, Stefan A.
dc.contributor.authorMelchers, Willem J.G.
dc.contributor.authorPeters, Cas J.A.
dc.contributor.authorToonen, Marga
dc.contributor.authorMcHugh, Martin P.
dc.contributor.authorTempleton, Kate E.
dc.contributor.authorClaas, Eric C.J.
dc.date.accessioned2020-11-23T15:30:35Z
dc.date.available2020-11-23T15:30:35Z
dc.date.issued2020-05-26
dc.identifier270075358
dc.identifier89211704-d43f-4290-9419-1203fcf5372c
dc.identifier85085534497
dc.identifier.citationBoers , S A , Melchers , W J G , Peters , C J A , Toonen , M , McHugh , M P , Templeton , K E & Claas , E C J 2020 , ' Multicenter evaluation of the QIAstat-Dx® Respiratory Panel V2 for the detection of viral and bacterial respiratory pathogens ' , Journal of Clinical Microbiology , vol. 58 , no. 6 , e01793-19 . https://doi.org/10.1128/jcm.01793-19en
dc.identifier.issn0095-1137
dc.identifier.otherBibtex: Boers_2020
dc.identifier.otherORCID: /0000-0002-0370-3700/work/80257887
dc.identifier.urihttps://hdl.handle.net/10023/21046
dc.description.abstractQIAstat-Dx Respiratory Panel V2 (RP) is a novel molecular-method-based syndromic test for the simultaneous and rapid (∼70-min) detection of 18 viral and 3 bacterial pathogens causing respiratory infections. This report describes the first multicenter retrospective comparison of the performance of the QIAstat-Dx RP assay to the established ePlex Respiratory Pathogen Panel (RPP) assay, for which we used 287 respiratory samples from patients suspected with respiratory infections. The QIAstat-Dx RP assay detected 312 (92%) of the 338 respiratory targets that were detected by the ePlex RPP assay. Most of the discrepant results have been observed in the low-pathogen-load samples. In addition, the QIAstat-Dx RP assay detected 19 additional targets in 19 respiratory samples that were not detected by the ePlex RPP assay. Nine of these discordant targets were considered to represent true positives after discrepancy testing by a third method. The main advantage of the QIAstat-Dx system compared to other syndromic testing systems, including the ePlex RPP assay, is the ability to generate cycle threshold (CT) values, which could help with the interpretation of results. Taking the data together, this study showed good performance of the QIAstat-Dx RP assay in comparison to the ePlex RPP assay for the detection of respiratory pathogens. The QIAstat-Dx RP assay offers a new, rapid, and accurate sample-to-answer multiplex panel for the detection of the most common viral and bacterial respiratory pathogens and therefore has the potential to direct appropriate therapy and infection control precautions.
dc.format.extent8
dc.format.extent302224
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Microbiologyen
dc.subjectQIAstat-Dxen
dc.subjectePlexen
dc.subjectMolecular diagnosticsen
dc.subjectRespiratory pathogen panelen
dc.subjectRespiratory tract infectionsen
dc.subjectSyndromic testingen
dc.subjectQR355 Virologyen
dc.subjectRB Pathologyen
dc.subjectDASen
dc.subject.lccQR355en
dc.subject.lccRBen
dc.titleMulticenter evaluation of the QIAstat-Dx® Respiratory Panel V2 for the detection of viral and bacterial respiratory pathogensen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.identifier.doi10.1128/jcm.01793-19
dc.description.statusPeer revieweden


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