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The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model
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dc.contributor.author | Moynié, Lucile | |
dc.contributor.author | Milenkovic, Stefan | |
dc.contributor.author | Mislin, Gaëtan L. A. | |
dc.contributor.author | Gasser, Véronique | |
dc.contributor.author | Malloci, Giuliano | |
dc.contributor.author | Baco, Etienne | |
dc.contributor.author | McCaughan, Rory P. | |
dc.contributor.author | Page, Malcolm G. P. | |
dc.contributor.author | Schalk, Isabelle J. | |
dc.contributor.author | Ceccarelli, Matteo | |
dc.contributor.author | Naismith, James H. | |
dc.date.accessioned | 2020-11-17T15:30:20Z | |
dc.date.available | 2020-11-17T15:30:20Z | |
dc.date.issued | 2019-08-14 | |
dc.identifier.citation | Moynié , L , Milenkovic , S , Mislin , G L A , Gasser , V , Malloci , G , Baco , E , McCaughan , R P , Page , M G P , Schalk , I J , Ceccarelli , M & Naismith , J H 2019 , ' The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model ' , Nature Communications , vol. 10 , 3673 . https://doi.org/10.1038/s41467-019-11508-y | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | PURE: 271270654 | |
dc.identifier.other | PURE UUID: 9a1596e6-04f8-4d6b-bd60-9ed874dea49d | |
dc.identifier.other | Jisc: 504475dcb5814d73956fa63159751fce | |
dc.identifier.other | publisher-id: s41467-019-11508-y | |
dc.identifier.other | manuscript: 11508 | |
dc.identifier.other | Scopus: 85070779528 | |
dc.identifier.other | WOS: 000480683300003 | |
dc.identifier.uri | https://hdl.handle.net/10023/20995 | |
dc.description | The research benefitted from support from ND4BB ENABLE Consortium has received support from the Innovative Medicines Initiatives Joint Undertaking under Grant Agreement nos. 115525 and 115583, resources which are composed of financial contribution from the European Union’s seventh framework programme (FP7/ 2007–2013) and EFPIA companies in kind contribution. This is work is supported by a Wellcome Trust Investigator (100209/Z/12/Z) award. M.C. and S.M. thank the additional financial support of MIUR with the PRIN project 2015795S5W_005. I.S., G.L.A.M., V.G. and E.B. thank also Vaincre la Mucoviscidose and Association Gregory Lemarchal, French associations against cystic fibrosis for additional financial support. J.H.N. and L.M. thank the Membrane Protein Laboratory at Diamond for beam time access. | en |
dc.description.abstract | Bacteria use small molecules called siderophores to scavenge iron. Siderophore-Fe3+ complexes are recognised by outer-membrane transporters and imported into the periplasm in a process dependent on the inner-membrane protein TonB. The siderophore enterobactin is secreted by members of the family Enterobacteriaceae, but many other bacteria including Pseudomonas species can use it. Here, we show that the Pseudomonas transporter PfeA recognises enterobactin using extracellular loops distant from the pore. The relevance of this site is supported by in vivo and in vitro analyses. We suggest there is a second binding site deeper inside the structure and propose that correlated changes in hydrogen bonds link binding-induced structural re-arrangements to the structural adjustment of the periplasmic TonB-binding motif. | |
dc.format.extent | 14 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Communications | en |
dc.rights | Copyright © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.subject | QR Microbiology | en |
dc.subject | DAS | en |
dc.subject.lcc | QR | en |
dc.title | The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.identifier.doi | https://doi.org/10.1038/s41467-019-11508-y | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | 100209/Z/12/Z | en |
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