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dc.contributor.authorBower, Neil I.
dc.contributor.authorJohnston, Ian A.
dc.date.accessioned2011-12-05T17:03:59Z
dc.date.available2011-12-05T17:03:59Z
dc.date.issued2009-08-10
dc.identifier.citationBower , N I & Johnston , I A 2009 , ' Selection of reference genes for expression studies with fish myogenic cell cultures ' BMC Molecular Biology , vol. 10 , 80 , pp. - . https://doi.org/10.1186/1471-2199-10-80en
dc.identifier.issn1471-2199
dc.identifier.otherPURE: 15933941
dc.identifier.otherPURE UUID: 47b6d3eb-a7be-4063-ab1b-64100b68ab71
dc.identifier.otherWOS: 000269645700001
dc.identifier.otherScopus: 69849087745
dc.identifier.otherORCID: /0000-0002-7796-5754/work/47136017
dc.identifier.urihttp://hdl.handle.net/10023/2093
dc.description.abstractBackground: Relatively few studies have used cell culture systems to investigate gene expression and the regulation of myogenesis in fish. To produce robust data from quantitative real-time PCR mRNA levels need to be normalised using internal reference genes which have stable expression across all experimental samples. We have investigated the expression of eight candidate genes to identify suitable reference genes for use in primary myogenic cell cultures from Atlantic salmon (Salmo salar L.). The software analysis packages geNorm, Normfinder and Best keeper were used to rank genes according to their stability across 42 samples during the course of myogenic differentiation. Results: Initial results showed several of the candidate genes exhibited stable expression throughout myogenic culture while Sdha was identified as the least stable gene. Further analysis with geNorm, Normfinder and Bestkeeper identified Ef1 alpha, Hprt1, Ppia and RNApolII as stably expressed. Comparison of data normalised with the geometric average obtained from combinations of any three of these genes showed no significant differences, indicating that any combination of these genes is valid. Conclusion: The geometric average of any three of Hprt1, Ef1 a, Ppia and RNApolII is suitable for normalisation of gene expression data in primary myogenic cultures from Atlantic salmon.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofBMC Molecular Biologyen
dc.rights© 2009 Bower and Johnston; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectReal-time PCRen
dc.subjectDifferential expressionen
dc.subjectHousekeeping genesen
dc.subjectSkeletal-muscleen
dc.subjectMyoblast fusionen
dc.subjectBeta-actinen
dc.subjectIn-vitroen
dc.subjectRT-PCRen
dc.subjectMessengeren
dc.subjectRNAen
dc.subjectQH426 Geneticsen
dc.subject.lccQH426en
dc.titleSelection of reference genes for expression studies with fish myogenic cell culturesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews.Centre for Research into Ecological & Environmental Modellingen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.identifier.doihttps://doi.org/10.1186/1471-2199-10-80
dc.description.statusPeer revieweden


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