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dc.contributor.authorGusachenko, Olesya Nikolaevna
dc.contributor.authorWoodford, Luke
dc.contributor.authorBalbirnie-Cumming, Katharin
dc.contributor.authorRyabov, Eugene V.
dc.contributor.authorEvans, David John
dc.date.accessioned2020-10-16T16:30:02Z
dc.date.available2020-10-16T16:30:02Z
dc.date.issued2020-10-08
dc.identifier.citationGusachenko , O N , Woodford , L , Balbirnie-Cumming , K , Ryabov , E V & Evans , D J 2020 , ' Evidence for and against deformed wing virus spillover from honey bees to bumble bees : a reverse genetic analysis ' , Scientific Reports , vol. 10 , 16847 . https://doi.org/10.1038/s41598-020-73809-3en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 270294697
dc.identifier.otherPURE UUID: 50a0e64d-99cf-4dcf-beeb-021fae317f80
dc.identifier.otherORCID: /0000-0003-2530-2120/work/82179738
dc.identifier.urihttp://hdl.handle.net/10023/20792
dc.descriptionFunding: This work was supported by grant funding from BBSRC BB/M00337X/2 and BB/I000828/1. This research was also supported by the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) grant 2017-06481 (EVR).en
dc.description.abstractDeformed wing virus (DWV) is a persistent pathogen of European honey bees and the major contributor to overwintering colony losses. The prevalence of DWV in honey bees has led to significant concerns about spillover of the virus to other pollinating species. Bumble bees are both a major group of wild and commercially-reared pollinators. Several studies have reported pathogen spillover of DWV from honey bees to bumble bees, but evidence of a sustained viral infection characterized by virus replication and accumulation has yet to be demonstrated. Here we investigate the infectivity and transmission of DWV in bumble bees using the buff-tailed bumble bee Bombus terrestris as a model. We apply a reverse genetics approach combined with controlled laboratory conditions to detect and monitor DWV infection. A novel reverse genetics system for three representative DWV variants, including the two master variants of DWV - type A and B - was used. Our results directly confirm DWV replication in bumble bees but also demonstrate striking resistance to infection by certain transmission routes. Bumble bees may support DWV replication but it is not clear how infection could occur under natural environmental conditions.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en
dc.subjectViral pathogenesisen
dc.subjectViral reservoirsen
dc.subjectViral transmissionen
dc.subjectQR355 Virologyen
dc.subjectNDASen
dc.subject.lccQR355en
dc.titleEvidence for and against deformed wing virus spillover from honey bees to bumble bees : a reverse genetic analysisen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/s41598-020-73809-3
dc.description.statusPeer revieweden


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