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dc.contributor.authorCalero, Patricia
dc.contributor.authorVolke, Daniel C.
dc.contributor.authorLowe, Phillip
dc.contributor.authorGotfredsen, Charlotte H.
dc.contributor.authorO'Hagan, David
dc.contributor.authorNikel, Pablo I.
dc.identifier.citationCalero , P , Volke , D C , Lowe , P , Gotfredsen , C H , O'Hagan , D & Nikel , P I 2020 , ' A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida ' , Nature Communications , vol. 11 , 5045 .
dc.identifier.otherPURE: 270460359
dc.identifier.otherPURE UUID: 510b9b3b-df50-4c2a-bd43-f7ef33aa6213
dc.identifier.otherORCID: /0000-0002-0510-5552/work/82179243
dc.identifier.otherScopus: 85092283922
dc.identifier.otherWOS: 000577244600011
dc.identifier.otherORCID: /0000-0003-1568-0750/work/122719949
dc.descriptionFunding: This work was supported by grants from The Novo Nordisk Foundation (NNF10CC1016517 and NNF18OC0034818), the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 814418 (SinFonia) and the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B) to P.I.N. We also thank the NMR Center at the Technical University of Denmark and the Villum Foundation for facilitating access to the 600 and 800 MHz NMR spectrometers.en
dc.description.abstractFluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and approaches to integrate fluorometabolites into the biochemistry of living cells are scarce. In this work, synthetic gene circuits for organofluorine biosynthesis are implemented in the platform bacterium Pseudomonas putida. By harnessing fluoride-responsive riboswitches and the orthogonal T7 RNA polymerase, biochemical reactions needed for in vivo biofluorination are wired to the presence of fluoride (i.e. circumventing the need of feeding expensive additives). Biosynthesis of fluoronucleotides and fluorosugars in engineered P. putida is demonstrated with mineral fluoride both as only fluorine source (i.e. substrate of the pathway) and as inducer of the synthetic circuit. This approach expands the chemical landscape of cell factories by providing alternative biosynthetic strategies towards fluorinated building-blocks.
dc.relation.ispartofNature Communicationsen
dc.rightsCopyright © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectQD Chemistryen
dc.subjectQR Microbiologyen
dc.titleA fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putidaen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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