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dc.contributor.authorKeep, Sarah
dc.contributor.authorOade, Michael S
dc.contributor.authorLidzbarski-Silvestre, Filip
dc.contributor.authorBentley, Kirsten
dc.contributor.authorStevenson-Leggett, Phoebe
dc.contributor.authorFreimanis, Graham L
dc.contributor.authorTennakoon, Chandana
dc.contributor.authorSanderson, Nicholas
dc.contributor.authorHammond, John A
dc.contributor.authorJones, Richard C
dc.contributor.authorBritton, Paul
dc.contributor.authorBickerton, Erica
dc.identifier.citationKeep , S , Oade , M S , Lidzbarski-Silvestre , F , Bentley , K , Stevenson-Leggett , P , Freimanis , G L , Tennakoon , C , Sanderson , N , Hammond , J A , Jones , R C , Britton , P & Bickerton , E 2020 , ' Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus ' , Journal of General Virology .
dc.identifier.otherORCID: /0000-0002-6619-2098/work/80257873
dc.descriptionFunding: This work was supported by Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/L003988/1 and 1645891, and strategic funding to The Pirbright Institute, BBS/E/I/00007035, BBS/E/I/00007034, BBS/E/I/00007037 and BBS/E/I/00007039.en
dc.description.abstractCoronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5' untranslated region (UTR), and the other upstream of each structural and accessory gene (TRS-B). Several coronaviruses have an ORF located between the N gene and the 3'-UTR, an area previously thought to be non-coding in the Gammacoronavirus infectious bronchitis virus (IBV) due to a lack of a canonical TRS-B. Here, we identify a non-canonical TRS-B allowing for a novel sgmRNA relating to this ORF to be produced in several strains of IBV: Beaudette, CR88, H120, D1466, Italy-02 and QX. Interestingly, the potential protein produced by this ORF is prematurely truncated in the Beaudette strain. A single nucleotide deletion was made in the Beaudette strain allowing for the generation of a recombinant IBV (rIBV) that had the potential to express a full-length protein. Assessment of this rIBV in vitro demonstrated that restoration of the full-length potential protein had no effect on viral replication. Further assessment of the Beaudette-derived RNA identified a second non-canonically transcribed sgmRNA located within gene 2. Deep sequencing analysis of allantoic fluid from Beaudette-infected embryonated eggs confirmed the presence of both the newly identified non-canonically transcribed sgmRNAs and highlighted the potential for further yet unidentified sgmRNAs. This HiSeq data, alongside the confirmation of non-canonically transcribed sgmRNAs, indicates the potential of the coronavirus genome to encode a larger repertoire of genes than has currently been identified.
dc.relation.ispartofJournal of General Virologyen
dc.subjectSub-genomic mRNAen
dc.subjectRNA synthesisen
dc.subjectQH301 Biologyen
dc.subjectQR355 Virologyen
dc.titleMultiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virusen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.description.statusPeer revieweden

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