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dc.contributor.authorRodríguez-Sevilla, Paloma
dc.contributor.authorSanz-Rodríguez, Francisco
dc.contributor.authorPeláez, Raúl P.
dc.contributor.authorDelgado-Buscalioni, Rafael
dc.contributor.authorLiang, Liangliang
dc.contributor.authorLiu, Xiaogang
dc.contributor.authorJaque, Daniel
dc.date.accessioned2020-09-15T23:37:22Z
dc.date.available2020-09-15T23:37:22Z
dc.date.issued2019-10-15
dc.identifier.citationRodríguez-Sevilla , P , Sanz-Rodríguez , F , Peláez , R P , Delgado-Buscalioni , R , Liang , L , Liu , X & Jaque , D 2019 , ' Upconverting nanorockers for intracellular viscosity measurements during chemotherapy ' , Advanced Biosystems , vol. 3 , no. 10 , 1900082 . https://doi.org/10.1002/adbi.201900082en
dc.identifier.issn2366-7478
dc.identifier.otherPURE: 261241947
dc.identifier.otherPURE UUID: a8c79e90-9ec9-4020-8dbe-9fb4a0effd2d
dc.identifier.otherRIS: urn:7CF0FF287F2D4044E759E1C23A6A3CFA
dc.identifier.otherORCID: /0000-0003-4743-6576/work/61979030
dc.identifier.otherScopus: 85073554444
dc.identifier.otherWOS: 000486722000001
dc.identifier.urihttp://hdl.handle.net/10023/20625
dc.descriptionFunding: Ministerio de Economía y Competitividad de España (MAT2016-75362-C3-1-R and MAT2017-83111R); Instituto de Salud Carlos III (PI16/ 00812); Comunidad Autónoma de Madrid (B2017/BMD-3867RENIMCM), and co-financed by the European Structural funds and also by COST action CM1403; Comunidad de Madrid predoctorate contracts and from the Spanish national project FIS2017-86007-C3-1-P (R.D-B and R.P.P).en
dc.description.abstractChemicals capable of producing structural and chemical changes on cells are used to treat diseases (e.g., cancer). Further development and optimization of chemotherapies require thorough knowledge of the effect of the chemical on the cellular structure and dynamics. This involves studying, in a noninvasive way, the properties of individual cells after drug administration. Intracellular viscosity is affected by chemical treatments and it can be reliably used to monitor chemotherapies at the cellular level. Here, cancer cell monitoring during chemotherapeutic treatments is demonstrated using intracellular allocated upconverting nanorockers. A simple analysis of the polarized visible emission of a single particle provides a real-time readout of its rocking dynamics that are directly correlated to the cytoplasmic viscosity. Numerical simulations and immunodetection are used to correlate the measured intracellular viscosity alterations to the changes produced in the cytoskeleton of cancer cells by anticancer drugs (colchicine and Taxol). This study evidences the possibility of monitoring cellular properties under an external chemical stimulus for the study and development of new treatments. Moreover, it provides the biomedical community with new tools to study intracellular dynamics and cell functioning.
dc.language.isoeng
dc.relation.ispartofAdvanced Biosystemsen
dc.rightsCopyright © 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1002/adbi.201900082en
dc.subjectCanceren
dc.subjectIn vitro chemotherapyen
dc.subjectRheometryen
dc.subjectUpconverting nanoparticlesen
dc.subjectQC Physicsen
dc.subjectQH301 Biologyen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectDASen
dc.subject.lccQCen
dc.subject.lccQH301en
dc.subject.lccRC0254en
dc.subject.lccRMen
dc.titleUpconverting nanorockers for intracellular viscosity measurements during chemotherapyen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.identifier.doihttps://doi.org/10.1002/adbi.201900082
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-09-16


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