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In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity
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dc.contributor.author | Kapsalis, Charalampos | |
dc.contributor.author | Ma, Yue | |
dc.contributor.author | Bode, Bela E. | |
dc.contributor.author | Pliotas, Christos | |
dc.date.accessioned | 2020-07-24T16:30:07Z | |
dc.date.available | 2020-07-24T16:30:07Z | |
dc.date.issued | 2020-07-21 | |
dc.identifier.citation | Kapsalis , C , Ma , Y , Bode , B E & Pliotas , C 2020 , ' In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity ' , Biophysical Journal , vol. 119 , no. 2 , pp. 448-459 . https://doi.org/10.1016/j.bpj.2020.06.012 | en |
dc.identifier.issn | 0006-3495 | |
dc.identifier.other | PURE: 268446005 | |
dc.identifier.other | PURE UUID: 7bfa0e14-e41f-4f1f-ae9c-9eb467c53a97 | |
dc.identifier.other | ORCID: /0000-0002-4309-4858/work/75610334 | |
dc.identifier.other | ORCID: /0000-0002-3384-271X/work/75610359 | |
dc.identifier.other | Scopus: 85087374078 | |
dc.identifier.other | WOS: 000552027100020 | |
dc.identifier.uri | https://hdl.handle.net/10023/20333 | |
dc.description | This project was supported by a BBSRC grant (BB/S018069/1) to C.P., who was supported by the Royal Society of Edinburgh, Tenovus (T15/41) and Carnegie Trust (OS000256), at the initial stages of this project. Further C.P. acknowledges support from the University of St Andrews for the C.K. studentship and the University of Leeds and the Chinese Scholarship Council for the Y.M. studentship. B.E.B. and C.P. acknowledge support by the Leverhulme Trust (RPG-2018–397). This work was also supported by previous Wellcome Trust [099149/Z/12/Z] and BBSRC equipment grants (BB/R013780/1). | en |
dc.description.abstract | The mechanosensitive channel of large conductance (MscL) from Mycobacterium tuberculosis has been used as structural model for rationalizing functional observations in multiple MscL orthologues. Although these orthologues adopt similar structural architectures, they reportedly present significant functional differences. Subtle structural discrepancies on mechanosensitive channel nano-pockets are known to affect mechanical gating and may be linked to large variability in tension sensitivity among these membrane channels. Here we modify the nano-pocket regions of MscL from Escherichia coli and Mycobacterium tuberculosis and employ PELDOR/DEER distance and 3pESEEM deuterium accessibility measurements to interrogate channel structure within lipids, in which both channels adopt a closed conformation. Significant in-lipid structural differences between the two constructs suggest a more compact EcMscL at the membrane inner-leaflet, as a consequence of a rotated TM2 helix. Observed differences within lipids could explain EcMscL’s higher tension sensitivity and should be taken into account in extrapolated models used for MscL gating rationalization. | |
dc.language.iso | eng | |
dc.relation.ispartof | Biophysical Journal | en |
dc.rights | Copyright © 2020 Biophysical Society. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/). | en |
dc.subject | QD Chemistry | en |
dc.subject | DAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QD | en |
dc.title | In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Leverhulme Trust | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Centre of Magnetic Resonance | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.identifier.doi | https://doi.org/10.1016/j.bpj.2020.06.012 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | RPG-2018-397 | en |
dc.identifier.grantnumber | BB/R013780/1 | en |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
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