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dc.contributor.authorPokhare, Somnath S.
dc.contributor.authorThorpe, Peter
dc.contributor.authorHedley, Pete
dc.contributor.authorMorris, Jennifer
dc.contributor.authorHabash, Samer S.
dc.contributor.authorElashry, Abdelnaser
dc.contributor.authorEves-van den Akker, Sebastian
dc.contributor.authorGrundler, Florian M. W.
dc.contributor.authorJones, John
dc.date.accessioned2020-07-22T16:30:04Z
dc.date.available2020-07-22T16:30:04Z
dc.date.issued2020-08-17
dc.identifier.citationPokhare , S S , Thorpe , P , Hedley , P , Morris , J , Habash , S S , Elashry , A , Eves-van den Akker , S , Grundler , F M W & Jones , J 2020 , ' Signatures of adaptation to a monocot host in the plant-parasitic cyst nematode Heterodera sacchari ' , The Plant Journal , vol. 103 , no. 4 , pp. 1263-1274 . https://doi.org/10.1111/tpj.14910en
dc.identifier.issn0960-7412
dc.identifier.otherPURE: 268711334
dc.identifier.otherPURE UUID: 3d49e0fc-02a4-4307-bbc7-a15e1527312b
dc.identifier.otherScopus: 85088307639
dc.identifier.otherWOS: 000550895100001
dc.identifier.urihttp://hdl.handle.net/10023/20310
dc.descriptionSomnath Pokhare was supported by a fellowship provided by the Indian Council of Agricultural Research (ICAR), Government of India. This work was also supported by Royal Society International Exchanges award number IE150670 and by the Rural and Environmental Science and Analytical Services Division of the Scottish Government. SEvdA is supported by Biotechnology and Biological Sciences Research Council grants BB/R011311/1 and BB/S006397/1. This work benefited from interactions funded through COST Action FA1208. The authors thank Dr Danny Coyne (IITA) for providing the starter culture of the H. sacchari population used for this work. Bioinformatics and Computational Biology analyses were supported by the University of St Andrews Bioinformatics Unit which is funded by a Wellcome Trust ISSF award [grant 105621/Z/14/Z].en
dc.description.abstractInteractions between plant‐parasitic nematodes and their hosts are mediated by effectors, i.e. secreted proteins that manipulate the plant to the benefit of the pathogen. To understand the role of effectors in host adaptation in nematodes, we analysed the transcriptome of Heterodera sacchari , a cyst nematode parasite of rice (Oryza sativa ) and sugarcane (Saccharum officinarum ). A multi‐gene phylogenetic analysis showed that H. sacchari and the cereal cyst nematode Heterodera avenae share a common evolutionary origin and that they evolved to parasitise monocot plants from a common dicot‐parasitic ancestor. We compared the effector repertoires of H. sacchari with those of the dicot parasites Heterodera glycines and Globodera rostochiensis to understand the consequences of this transition. While, in general, effector repertoires are similar between the species, comparing effectors and non‐effectors of H. sacchari and G. rostochiensis shows that effectors have accumulated more mutations than non‐effectors. Although most effectors show conserved spatiotemporal expression profiles and likely function, some H. sacchari effectors are adapted to monocots. This is exemplified by the plant‐peptide hormone mimics, the CLAVATA3/EMBRYO SURROUNDING REGION‐like (CLE) effectors. Peptide hormones encoded by H. sacchari CLE effectors are more similar to those from rice than those from other plants, or those from other plant‐parasitic nematodes. We experimentally validated the functional significance of these observations by demonstrating that CLE peptides encoded by H. sacchari induce a short root phenotype in rice, whereas those from a related dicot parasite do not. These data provide a functional example of effector evolution that co‐occurred with the transition from a dicot‐parasitic to a monocot‐parasitic lifestyle.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofThe Plant Journalen
dc.rightsCopyright © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectPeptide hormone mimicsen
dc.subjectEffectorsen
dc.subjectHeterodera saccharien
dc.subjectTranscriptomicsen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccQH301en
dc.titleSignatures of adaptation to a monocot host in the plant-parasitic cyst nematode Heterodera saccharien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.1111/tpj.14910
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-07-22


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