Coupled differentiation and division of embryonic stem cells inferred from clonal snapshots
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The deluge of single-cell data obtained by sequencing, imaging and epigenetic markers has led to an increasingly detailed description of cell state. However, it remains challenging to identify how cells transition between different states, in part because data are typically limited to snapshots in time. A prerequisite for inferring cell state transitions from such snapshots is to distinguish whether transitions are coupled to cell divisions. To address this, we present two minimal branching process models of cell division and differentiation in a well-mixed population. These models describe dynamics where differentiation and division are coupled or uncoupled. For each model, we derive analytic expressions for each subpopulation's mean and variance and for the likelihood, allowing exact Bayesian parameter inference and model selection in the idealised case of fully observed trajectories of differentiation and division events. In the case of snapshots, we present a sample path algorithm and use this to predict optimal temporal spacing of measurements for experimental design. We then apply this methodology to an in vitro dataset assaying the clonal growth of epiblast stem cells in culture conditions promoting self-renewal or differentiation. Here, the larger number of cell states necessitates approximate Bayesian computation. For both culture conditions, our inference supports the model where cell state transitions are coupled to division. For culture conditions promoting differentiation, our analysis indicates a possible shift in dynamics, with these processes becoming more coupled over time.
Ruske , L J , Kursawe , J , Tsakiridis , A , Wilson , V , Fletcher , A , Blythe , R A & Schumacher , L J 2020 , ' Coupled differentiation and division of embryonic stem cells inferred from clonal snapshots ' , Physical Biology , vol. 17 , no. 6 , 065009 . https://doi.org/10.1088/1478-3975/aba041
Copyright © 2020 The Author(s). Published by IOP Publishing Ltd. Open Access. Original content from this work may be used under the terms of the Creative Commons Attribution 4.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
DescriptionAGF was supported by a Vice-Chancellor's Fellowship from the University of Sheffield, LJS was supported by a Chancellor's Fellowship from the University of Edinburgh.
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