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dc.contributor.authorBroadhead, Matthew James
dc.contributor.authorHorrocks, Mathew
dc.contributor.authorZhu, Fei
dc.contributor.authorMuresan, Leila
dc.contributor.authorBenavides-Piccione, Ruth
dc.contributor.authorDeFelipe, Javier
dc.contributor.authorFricker, David
dc.contributor.authorKopanitsa, Maksym V.
dc.contributor.authorDuncan, Rory R.
dc.contributor.authorKlenerman, David
dc.contributor.authorKomiyama, Noboru H.
dc.contributor.authorLee, Steven F.
dc.contributor.authorGrant, Seth G. N.
dc.identifier.citationBroadhead , M J , Horrocks , M , Zhu , F , Muresan , L , Benavides-Piccione , R , DeFelipe , J , Fricker , D , Kopanitsa , M V , Duncan , R R , Klenerman , D , Komiyama , N H , Lee , S F & Grant , S G N 2016 , ' PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits ' , Scientific Reports , vol. 6 , 24626 .
dc.identifier.otherPURE: 268444417
dc.identifier.otherPURE UUID: eb4d0f25-837a-425a-aadf-e2a34375cbf2
dc.identifier.otherScopus: 84964619961
dc.descriptionSupport from the Wellcome Trust, Medical Research Council, European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements no. 604102 (HBP), 241995 (GENCODYS), 242498 (EUROSPIN), 242167 (SYNSYS).en
dc.description.abstractThe molecular features of synapses in the hippocampus underpin current models of learning and cognition. Although synapse ultra-structural diversity has been described in the canonical hippocampal circuitry, our knowledge of sub-synaptic organisation of synaptic molecules remains largely unknown. To address this, mice were engineered to express Post Synaptic Density 95 protein (PSD95) fused to either eGFP or mEos2 and imaged with two orthogonal super-resolution methods: gated stimulated emission depletion (g-STED) microscopy and photoactivated localisation microscopy (PALM). Large-scale analysis of ~100,000 synapses in 7 hippocampal sub-regions revealed they comprised discrete PSD95 nanoclusters that were spatially organised into single and multi-nanocluster PSDs. Synapses in different sub-regions, cell-types and locations along the dendritic tree of CA1 pyramidal neurons, showed diversity characterised by the number of nanoclusters per synapse. Multi-nanocluster synapses were frequently found in the CA3 and dentate gyrus sub-regions, corresponding to large thorny excrescence synapses. Although the structure of individual nanoclusters remained relatively conserved across all sub-regions, PSD95 packing into nanoclusters also varied between sub-regions determined from nanocluster fluorescence intensity. These data identify PSD95 nanoclusters as a basic structural unit, or building block, of excitatory synapses and their number characterizes synapse size and structural diversity.
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright © 2016 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.titlePSD95 nanoclusters are postsynaptic building blocks in hippocampus circuitsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Psychology and Neuroscienceen
dc.description.statusPeer revieweden

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