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dc.contributor.authorVeettikazhy, Madhu
dc.contributor.authorNylk, Jonathan
dc.contributor.authorGasparoli, Federico
dc.contributor.authorEscobet-Montalbán, Adrià
dc.contributor.authorHansen, Anders Kragh
dc.contributor.authorMarti, Dominik
dc.contributor.authorAndersen, Peter Eskil
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2020-05-26T10:30:13Z
dc.date.available2020-05-26T10:30:13Z
dc.date.issued2020-05-15
dc.identifier268156695
dc.identifier2a29a04a-18c6-49df-a877-cd00453e2cd8
dc.identifier85084786005
dc.identifier000562212900002
dc.identifier.citationVeettikazhy , M , Nylk , J , Gasparoli , F , Escobet-Montalbán , A , Hansen , A K , Marti , D , Andersen , P E & Dholakia , K 2020 , ' Multi-photon attenuation-compensated light-sheet fluorescence microscopy ' , Scientific Reports , vol. 10 , 8090 . https://doi.org/10.1038/s41598-020-64891-8en
dc.identifier.issn2045-2322
dc.identifier.otherRIS: urn:68E35D7D75DD6F5C34189ABB394CFF3D
dc.identifier.otherRIS: Veettikazhy2020
dc.identifier.otherORCID: /0000-0002-2977-4929/work/74873028
dc.identifier.urihttps://hdl.handle.net/10023/19994
dc.descriptionWe thank the UK Engineering and Physical Sciences Research Council for funding (grants EP/P030017/1 and EP/R004854/1), the European Union’s Horizon 2020 Framework Programme (H2020) (675512, BE-OPTICAL), the Danish Council for Independent Research (DFF FTP grant 7017-00021), and the Otto Mønsted Foundation (grant 19-70-0109).en
dc.description.abstractAttenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, can be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A “gold standard” system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users.
dc.format.extent10
dc.format.extent3630304
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.subjectQC Physicsen
dc.subjectDASen
dc.subject.lccQCen
dc.titleMulti-photon attenuation-compensated light-sheet fluorescence microscopyen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1038/s41598-020-64891-8
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/P030017/1en
dc.identifier.grantnumberEP/R004854/1en
dc.identifier.grantnumber675512en


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