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Multi-photon attenuation-compensated light-sheet fluorescence microscopy
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dc.contributor.author | Veettikazhy, Madhu | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Gasparoli, Federico | |
dc.contributor.author | Escobet-Montalbán, Adrià | |
dc.contributor.author | Hansen, Anders Kragh | |
dc.contributor.author | Marti, Dominik | |
dc.contributor.author | Andersen, Peter Eskil | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2020-05-26T10:30:13Z | |
dc.date.available | 2020-05-26T10:30:13Z | |
dc.date.issued | 2020-05-15 | |
dc.identifier.citation | Veettikazhy , M , Nylk , J , Gasparoli , F , Escobet-Montalbán , A , Hansen , A K , Marti , D , Andersen , P E & Dholakia , K 2020 , ' Multi-photon attenuation-compensated light-sheet fluorescence microscopy ' , Scientific Reports , vol. 10 , 8090 . https://doi.org/10.1038/s41598-020-64891-8 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | PURE: 268156695 | |
dc.identifier.other | PURE UUID: 2a29a04a-18c6-49df-a877-cd00453e2cd8 | |
dc.identifier.other | RIS: urn:68E35D7D75DD6F5C34189ABB394CFF3D | |
dc.identifier.other | RIS: Veettikazhy2020 | |
dc.identifier.other | Scopus: 85084786005 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/74873028 | |
dc.identifier.other | WOS: 000562212900002 | |
dc.identifier.uri | https://hdl.handle.net/10023/19994 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council for funding (grants EP/P030017/1 and EP/R004854/1), the European Union’s Horizon 2020 Framework Programme (H2020) (675512, BE-OPTICAL), the Danish Council for Independent Research (DFF FTP grant 7017-00021), and the Otto Mønsted Foundation (grant 19-70-0109). | en |
dc.description.abstract | Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, can be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A “gold standard” system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users. | |
dc.format.extent | 10 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.rights | Copyright © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.subject | QC Physics | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.title | Multi-photon attenuation-compensated light-sheet fluorescence microscopy | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | European Commission | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1038/s41598-020-64891-8 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
dc.identifier.grantnumber | 675512 | en |
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