Files in this item
Multi-photon attenuation-compensated light-sheet fluorescence microscopy
Item metadata
dc.contributor.author | Veettikazhy, Madhu | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Gasparoli, Federico | |
dc.contributor.author | Escobet-Montalbán, Adrià | |
dc.contributor.author | Hansen, Anders Kragh | |
dc.contributor.author | Marti, Dominik | |
dc.contributor.author | Andersen, Peter Eskil | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2020-05-26T10:30:13Z | |
dc.date.available | 2020-05-26T10:30:13Z | |
dc.date.issued | 2020-05-15 | |
dc.identifier | 268156695 | |
dc.identifier | 2a29a04a-18c6-49df-a877-cd00453e2cd8 | |
dc.identifier | 85084786005 | |
dc.identifier | 000562212900002 | |
dc.identifier.citation | Veettikazhy , M , Nylk , J , Gasparoli , F , Escobet-Montalbán , A , Hansen , A K , Marti , D , Andersen , P E & Dholakia , K 2020 , ' Multi-photon attenuation-compensated light-sheet fluorescence microscopy ' , Scientific Reports , vol. 10 , 8090 . https://doi.org/10.1038/s41598-020-64891-8 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | RIS: urn:68E35D7D75DD6F5C34189ABB394CFF3D | |
dc.identifier.other | RIS: Veettikazhy2020 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/74873028 | |
dc.identifier.uri | https://hdl.handle.net/10023/19994 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council for funding (grants EP/P030017/1 and EP/R004854/1), the European Union’s Horizon 2020 Framework Programme (H2020) (675512, BE-OPTICAL), the Danish Council for Independent Research (DFF FTP grant 7017-00021), and the Otto Mønsted Foundation (grant 19-70-0109). | en |
dc.description.abstract | Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, can be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A “gold standard” system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users. | |
dc.format.extent | 10 | |
dc.format.extent | 3630304 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | QC Physics | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.title | Multi-photon attenuation-compensated light-sheet fluorescence microscopy | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1038/s41598-020-64891-8 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
dc.identifier.grantnumber | 675512 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.