Show simple item record

Files in this item


Item metadata

dc.contributor.authorGoswami, Cosmika
dc.contributor.authorFox, Stephen
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorConnor, Martin
dc.contributor.authorLeanord, Alistair
dc.contributor.authorEvans, Thomas J.
dc.identifier.citationGoswami , C , Fox , S , Holden , M T G , Connor , M , Leanord , A & Evans , T J 2020 , ' Origin, maintenance and spread of antibiotic resistance genes within plasmids and chromosomes of bloodstream isolates of Escherichia coli ' , Microbial Genomics , vol. 6 , no. 4 , 000353 .
dc.identifier.otherPURE: 268138082
dc.identifier.otherPURE UUID: 80a229d4-18ba-4a3a-a3de-5a81059f693b
dc.identifier.otherWOS: 000531010100006
dc.identifier.otherORCID: /0000-0002-4958-2166/work/74873043
dc.identifier.otherScopus: 85085042639
dc.descriptionThe work was funded by the Scottish Executive via the Chief Scientists Office through the provision of a grant to establish the Scottish Healthcare Associated Infection Prevention Institute (SHAIPI).en
dc.description.abstractBlood stream invasion by Escherichia coli is the commonest cause of bacteremia in the UK and elsewhere with an attributable mortality of about 15–20 %; antibiotic resistance to multiple agents is common in this microbe and is associated with worse outcomes. Genes conferring antimicrobial resistance, and their frequent location on horizontally transferred genetic elements is well-recognised, but the origin of these determinants, and their ability to be maintained and spread within clinically-relevant bacterial populations is unclear. Here, we set out to examine the distribution of antimicrobial resistance genes in chromosomes and plasmids of 16 bloodstream isolates of E. coli from patients within Scotland, and how these genes are maintained and spread. Using a combination of short and long-read whole genome sequencing methods, we were able to assemble complete sequences of 44 plasmids, with 16 Inc group F and 20 col plasmids; antibiotic resistance genes located almost exclusively within the F group. blaCTX-M15 genes had re-arranged in some strains into the chromosome alone (five strains), while others contained plasmid copies alone (two strains). Integrons containing multiple antibiotic genes were widespread in plasmids, notably many with a dfrA7 gene encoding resistance to trimethoprim, thus linking trimethoprim resistance to the other antibiotic resistance genes within the plasmids. This will allow even narrow spectrum antibiotics such as trimethoprim to act as a selective agent for plasmids containing antibiotic resistance genes mediating much broader resistance, including blaCTX-M15. To our knowledge, this is the first analysis to provide complete sequence data of chromosomes and plasmids in a collection of pathogenic human bloodstream isolates of E. coli. Our findings reveal the interplay between plasmids and integrative and conjugative elements in the maintenance and spread of antibiotic resistance genes within pathogenic E. coli.
dc.relation.ispartofMicrobial Genomicsen
dc.rightsCopyright © 2020 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License.en
dc.subjectExtended spectrum beta-lactamasesen
dc.subjectHorizontal gene transferen
dc.subjectQR180 Immunologyen
dc.titleOrigin, maintenance and spread of antibiotic resistance genes within plasmids and chromosomes of bloodstream isolates of Escherichia colien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record