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dc.contributor.authorBaron, Vincent O.
dc.contributor.authorChen, Mingzhou
dc.contributor.authorHammarstrom, Bjorn
dc.contributor.authorHammond, Robert J. H.
dc.contributor.authorGlynne-Jones, Peter
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2020-05-14T14:30:03Z
dc.date.available2020-05-14T14:30:03Z
dc.date.issued2020-05-14
dc.identifier.citationBaron , V O , Chen , M , Hammarstrom , B , Hammond , R J H , Glynne-Jones , P , Gillespie , S H & Dholakia , K 2020 , ' Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform ' , Communications Biology , vol. 3 , 236 . https://doi.org/10.1038/s42003-020-0915-3en
dc.identifier.issn2399-3642
dc.identifier.otherPURE: 266146731
dc.identifier.otherPURE UUID: fadf8ee4-940b-4bca-8d68-58404ff5a587
dc.identifier.otherORCID: /0000-0001-6537-7712/work/74117943
dc.identifier.otherORCID: /0000-0002-6190-5167/work/74117952
dc.identifier.otherScopus: 85084703784
dc.identifier.otherWOS: 000618915300001
dc.identifier.urihttps://hdl.handle.net/10023/19942
dc.descriptionFunding: UK Engineering and Physical Sciences Research Council for funding through grant EP/P030017/1 and fellowship EP/L025035/1. This work was also supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007–2013) and EFPIA companies’ in kind contribution (www.imi.europa.eu)] and the PanACEA consortium [funded by the European & Developing Countries Clinical Trials Partnership (EDCTP); grant agreement: TRIA-2015-1102]en
dc.description.abstractTuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofCommunications Biologyen
dc.rightsCopyright © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectQR Microbiologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectDASen
dc.subjectBDCen
dc.subjectR2Cen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQRen
dc.subject.lccRMen
dc.titleReal-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platformen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/s42003-020-0915-3
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-05-14
dc.identifier.grantnumberEP/P030017/1en
dc.identifier.grantnumber115337en
dc.identifier.grantnumberen


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