Show simple item record

Files in this item


Item metadata

dc.contributor.authorBaron, Vincent O.
dc.contributor.authorChen, Mingzhou
dc.contributor.authorHammarstrom, Bjorn
dc.contributor.authorHammond, Robert J. H.
dc.contributor.authorGlynne-Jones, Peter
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorDholakia, Kishan
dc.identifier.citationBaron , V O , Chen , M , Hammarstrom , B , Hammond , R J H , Glynne-Jones , P , Gillespie , S H & Dholakia , K 2020 , ' Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform ' , Communications Biology , vol. 3 , 236 .
dc.identifier.otherORCID: /0000-0001-6537-7712/work/74117943
dc.identifier.otherORCID: /0000-0002-6190-5167/work/74117952
dc.identifier.otherORCID: /0000-0003-3664-3641/work/157140934
dc.descriptionFunding: UK Engineering and Physical Sciences Research Council for funding through grant EP/P030017/1 and fellowship EP/L025035/1. This work was also supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007–2013) and EFPIA companies’ in kind contribution (] and the PanACEA consortium [funded by the European & Developing Countries Clinical Trials Partnership (EDCTP); grant agreement: TRIA-2015-1102]en
dc.description.abstractTuberculosis (TB) remains a leading cause of death worldwide. Lipid rich, phenotypically antibiotic tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse and the need for long-term TB treatment. We present a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis. We then perform optical analysis in the form of wavelength modulated Raman spectroscopy (WMRS) on the trapped M. smegmatis for up to eight hours, and also in the presence of isoniazid (INH). The Raman fingerprints of M. smegmatis exposed to INH change substantially in comparison to the unstressed condition. Our work provides a real-time assessment of the impact of INH on the increase of lipids in these mycobacteria, which could render the cells more tolerant to antibiotics. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to different conditions and stimuli.
dc.relation.ispartofCommunications Biologyen
dc.subjectQR Microbiologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleReal-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platformen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record