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dc.contributor.authorCorsetti, Stella
dc.contributor.authorWijesinghe, Philip
dc.contributor.authorPoulton, Persephone Beatrice
dc.contributor.authorSakata, Shuzo
dc.contributor.authorVyas, Kushi
dc.contributor.authorHerrington, C Simon
dc.contributor.authorNylk, Jonathan
dc.contributor.authorGasparoli, Federico Maria
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2020-04-15T12:30:02Z
dc.date.available2020-04-15T12:30:02Z
dc.date.issued2020-04-15
dc.identifier.citationCorsetti , S , Wijesinghe , P , Poulton , P B , Sakata , S , Vyas , K , Herrington , C S , Nylk , J , Gasparoli , F M & Dholakia , K 2020 , ' Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution ' , OSA Continuum , vol. 3 , no. 4 , pp. 1068-1083 . https://doi.org/10.1364/OSAC.391644en
dc.identifier.issn2578-7519
dc.identifier.otherPURE: 266467675
dc.identifier.otherPURE UUID: 15e5834b-e644-4523-89ff-fe2cdf6e4f99
dc.identifier.otherORCID: /0000-0002-2977-4929/work/72360569
dc.identifier.otherORCID: /0000-0002-8378-7261/work/72360990
dc.identifier.otherWOS: 000527275200032
dc.identifier.otherScopus: 85097728971
dc.identifier.urihttps://hdl.handle.net/10023/19804
dc.descriptionFunding: UK Engineering and Physical Sciences Research Council (EP/P030017/1, EP/R004854/1).en
dc.description.abstractImaging across length scales and in depth has been an important pursuit of widefield optical imaging, promising to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light-sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets, and demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer's disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology and histopathology.
dc.format.extent16
dc.language.isoeng
dc.relation.ispartofOSA Continuumen
dc.rightsCopyright © 2020 the Author(s). Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.en
dc.subjectQC Physicsen
dc.subjectQH301 Biologyen
dc.subjectRB Pathologyen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQCen
dc.subject.lccQH301en
dc.subject.lccRBen
dc.subject.lccRC0321en
dc.titleWidefield light sheet microscopy using an Airy beam combined with deep-learning super-resolutionen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEPSRCen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1364/OSAC.391644
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/P030017/1en
dc.identifier.grantnumberEP/R004854/1en


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