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Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution
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dc.contributor.author | Corsetti, Stella | |
dc.contributor.author | Wijesinghe, Philip | |
dc.contributor.author | Poulton, Persephone Beatrice | |
dc.contributor.author | Sakata, Shuzo | |
dc.contributor.author | Vyas, Kushi | |
dc.contributor.author | Herrington, C Simon | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Gasparoli, Federico Maria | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2020-04-15T12:30:02Z | |
dc.date.available | 2020-04-15T12:30:02Z | |
dc.date.issued | 2020-04-15 | |
dc.identifier | 266467675 | |
dc.identifier | 15e5834b-e644-4523-89ff-fe2cdf6e4f99 | |
dc.identifier | 000527275200032 | |
dc.identifier | 85097728971 | |
dc.identifier.citation | Corsetti , S , Wijesinghe , P , Poulton , P B , Sakata , S , Vyas , K , Herrington , C S , Nylk , J , Gasparoli , F M & Dholakia , K 2020 , ' Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution ' , OSA Continuum , vol. 3 , no. 4 , pp. 1068-1083 . https://doi.org/10.1364/OSAC.391644 | en |
dc.identifier.issn | 2578-7519 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/72360569 | |
dc.identifier.other | ORCID: /0000-0002-8378-7261/work/72360990 | |
dc.identifier.uri | https://hdl.handle.net/10023/19804 | |
dc.description | Funding: UK Engineering and Physical Sciences Research Council (EP/P030017/1, EP/R004854/1). | en |
dc.description.abstract | Imaging across length scales and in depth has been an important pursuit of widefield optical imaging, promising to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light-sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets, and demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer's disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology and histopathology. | |
dc.format.extent | 16 | |
dc.format.extent | 5845049 | |
dc.language.iso | eng | |
dc.relation.ispartof | OSA Continuum | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | RB Pathology | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | DAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | RB | en |
dc.subject.lcc | RC0321 | en |
dc.title | Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1364/OSAC.391644 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
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