Files in this item
Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation
Item metadata
dc.contributor.author | Mamsen, Linn Salto | |
dc.contributor.author | Charkiewicz, Karol | |
dc.contributor.author | Anderson, Richard A. | |
dc.contributor.author | Telfer, Evelyn E. | |
dc.contributor.author | McLaughlin, Marie | |
dc.contributor.author | Kelsey, Thomas W. | |
dc.contributor.author | Kristensen, Stine G. | |
dc.contributor.author | Gook, Debra A. | |
dc.contributor.author | Ernst, Erik | |
dc.contributor.author | Andersen, Claus Yding | |
dc.date.accessioned | 2020-03-25T00:33:30Z | |
dc.date.available | 2020-03-25T00:33:30Z | |
dc.date.issued | 2019-06 | |
dc.identifier | 257593995 | |
dc.identifier | 88364590-4ad1-462c-8199-e6d94489d162 | |
dc.identifier | 000469444000033 | |
dc.identifier | 85063289657 | |
dc.identifier | 000469444000033 | |
dc.identifier.citation | Mamsen , L S , Charkiewicz , K , Anderson , R A , Telfer , E E , McLaughlin , M , Kelsey , T W , Kristensen , S G , Gook , D A , Ernst , E & Andersen , C Y 2019 , ' Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation ' , Fertility and Sterility , vol. 111 , no. 6 , pp. 1217-1225.e3 . https://doi.org/10.1016/j.fertnstert.2019.02.003 | en |
dc.identifier.issn | 0015-0282 | |
dc.identifier.other | ORCID: /0000-0002-8091-1458/work/55901217 | |
dc.identifier.uri | https://hdl.handle.net/10023/19703 | |
dc.description | Funding: Novo Nordic Foundation, the International EU project ReproUnion, Sofus Carl Emil og Hustrus Fond, and the Foundation for Polish Science (FNP). | en |
dc.description.abstract | Objective: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC). Design: Retrospective case-control study. Setting: University hospital. Patient(s): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer. Intervention(s): None. Main Outcome Measure(s): Follicle density (follicles/mm(3)), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro. Result(s): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimullerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%). Conclusion(s): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC. (C) 2019 by American Society for Reproductive Medicine. | |
dc.format.extent | 12 | |
dc.format.extent | 279660 | |
dc.language.iso | eng | |
dc.relation.ispartof | Fertility and Sterility | en |
dc.subject | Turner syndrome | en |
dc.subject | Follicle density | en |
dc.subject | Ovarian tissue cryopreservation | en |
dc.subject | QA75 Electronic computers. Computer science | en |
dc.subject | QH301 Biology | en |
dc.subject | RG Gynecology and obstetrics | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QA75 | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | RG | en |
dc.title | Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Computer Science | en |
dc.contributor.institution | University of St Andrews. Centre for Interdisciplinary Research in Computational Algebra | en |
dc.identifier.doi | 10.1016/j.fertnstert.2019.02.003 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2020-03-25 |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.