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dc.contributor.authorMamsen, Linn Salto
dc.contributor.authorCharkiewicz, Karol
dc.contributor.authorAnderson, Richard A.
dc.contributor.authorTelfer, Evelyn E.
dc.contributor.authorMcLaughlin, Marie
dc.contributor.authorKelsey, Thomas W.
dc.contributor.authorKristensen, Stine G.
dc.contributor.authorGook, Debra A.
dc.contributor.authorErnst, Erik
dc.contributor.authorAndersen, Claus Yding
dc.date.accessioned2020-03-25T00:33:30Z
dc.date.available2020-03-25T00:33:30Z
dc.date.issued2019-06
dc.identifier.citationMamsen , L S , Charkiewicz , K , Anderson , R A , Telfer , E E , McLaughlin , M , Kelsey , T W , Kristensen , S G , Gook , D A , Ernst , E & Andersen , C Y 2019 , ' Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation ' , Fertility and Sterility , vol. 111 , no. 6 , pp. 1217-1225.e3 . https://doi.org/10.1016/j.fertnstert.2019.02.003en
dc.identifier.issn0015-0282
dc.identifier.otherPURE: 257593995
dc.identifier.otherPURE UUID: 88364590-4ad1-462c-8199-e6d94489d162
dc.identifier.otherWOS: 000469444000033
dc.identifier.otherORCID: /0000-0002-8091-1458/work/55901217
dc.identifier.otherScopus: 85063289657
dc.identifier.otherWOS: 000469444000033
dc.identifier.urihttp://hdl.handle.net/10023/19703
dc.descriptionFunding: Novo Nordic Foundation, the International EU project ReproUnion, Sofus Carl Emil og Hustrus Fond, and the Foundation for Polish Science (FNP).en
dc.description.abstractObjective: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC). Design: Retrospective case-control study. Setting: University hospital. Patient(s): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer. Intervention(s): None. Main Outcome Measure(s): Follicle density (follicles/mm(3)), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro. Result(s): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimullerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%). Conclusion(s): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC. (C) 2019 by American Society for Reproductive Medicine.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofFertility and Sterilityen
dc.rightsCopyright © 2019 American Society for Reproductive Medicine, Published by Elsevier Inc. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.fertnstert.2019.02.003en
dc.subjectTurner syndromeen
dc.subjectFollicle densityen
dc.subjectOvarian tissue cryopreservationen
dc.subjectQA75 Electronic computers. Computer scienceen
dc.subjectQH301 Biologyen
dc.subjectRG Gynecology and obstetricsen
dc.subjectNDASen
dc.subject.lccQA75en
dc.subject.lccQH301en
dc.subject.lccRGen
dc.titleCharacterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservationen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Computer Scienceen
dc.contributor.institutionUniversity of St Andrews. Centre for Interdisciplinary Research in Computational Algebraen
dc.identifier.doihttps://doi.org/10.1016/j.fertnstert.2019.02.003
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-03-25


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