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dc.contributor.authorHall, Matthew D
dc.contributor.authorHolden, Matthew TG
dc.contributor.authorSrisomang, Pramot
dc.contributor.authorMahavanakul, Weera
dc.contributor.authorWuthiekanun, Vanaporn
dc.contributor.authorLimmathurotsakul, Direk
dc.contributor.authorFountain, Kay
dc.contributor.authorParkhill, Julian
dc.contributor.authorNickerson, Emma K
dc.contributor.authorPeacock, Sharon J
dc.contributor.authorFraser, Christophe
dc.identifier.citationHall , M D , Holden , M TG , Srisomang , P , Mahavanakul , W , Wuthiekanun , V , Limmathurotsakul , D , Fountain , K , Parkhill , J , Nickerson , E K , Peacock , S J & Fraser , C 2019 , ' Improved characterisation of MRSA transmission using within-host bacterial sequence diversity ' , eLife , vol. 8 , e46402 .
dc.identifier.otherPURE: 262880770
dc.identifier.otherPURE UUID: 9b7ed0d6-5d4e-44ac-a9d1-b119c83837be
dc.identifier.otherPubMed: 31591959
dc.identifier.otherORCID: /0000-0002-4958-2166/work/64361293
dc.identifier.otherScopus: 85074463963
dc.identifier.otherWOS: 000506842000001
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) transmission in the hospital setting has been a frequent subject of investigation using bacterial genomes, but previous approaches have not yet fully utilised the extra deductive power provided when multiple pathogen samples are acquired from each host. Here, we use a large dataset of MRSA sequences from multiply-sampled patients to reconstruct colonisation of individuals in a high-transmission setting in a hospital in Thailand. We reconstructed transmission trees for MRSA. We also investigated transmission between anatomical sites on the same individual, finding that this either occurs repeatedly or involves a wide transmission bottleneck. We examined the between-subject bottleneck, finding a wide range in the amount of diversity transmitted. Finally, we compared our approach to the simpler method of identifying transmission pairs using single nucleotide polymorphism (SNP) counts. This suggested that the optimum threshold for identifying a pair is 39 SNPs, if sensitivities and specificities are equally weighted.
dc.rightsCopyright © 2019 Hall et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en
dc.subjectQH426 Geneticsen
dc.subjectQR Microbiologyen
dc.subjectRB Pathologyen
dc.titleImproved characterisation of MRSA transmission using within-host bacterial sequence diversityen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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