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dc.contributor.authorJamieson, Lauren E.
dc.contributor.authorHarrison, David J.
dc.contributor.authorCampbell, Colin J.
dc.date.accessioned2020-01-09T00:37:42Z
dc.date.available2020-01-09T00:37:42Z
dc.date.issued2019-05
dc.identifier.citationJamieson , L E , Harrison , D J & Campbell , C J 2019 , ' Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine ' , Journal of Biophotonics , vol. 12 , no. 5 , e201800201 . https://doi.org/10.1002/jbio.201800201en
dc.identifier.issn1864-063X
dc.identifier.otherPURE: 256025534
dc.identifier.otherPURE UUID: 7a9f8f84-1c09-4d88-899c-89b80cb76d6b
dc.identifier.otherRIS: urn:2BD1F32D1DD4941682FA6EC66839FA8C
dc.identifier.otherScopus: 85059695843
dc.identifier.otherWOS: 000467052400004
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034214
dc.identifier.urihttp://hdl.handle.net/10023/19251
dc.description.abstractThere has been increasing use of in vitro cell culture models that more realistically replicate the three dimensional (3D) environment found in vivo. Multicellular tumour spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D 'sphere' that exhibits many of the characteristics found in vivo. Significantly, MTS develop gradients in nutrients and oxygen, commonly found in tumours, that contribute to therapy resistance. While MTS show promise as a more realistic in vitro culture model, there is a massive need to improve imaging technologies for assessing biochemical characteristics and drug response in such models to maximize their translation into useful applications such as high throughput screening (HTS). In this study we investigate the potential for Raman spectroscopy to unveil biochemical information in MTS and have investigated how spheroid age influences drug response, shedding light on increased therapy resistance in developing tumours. The wealth of molecular level information delivered by Raman spectroscopy in a noninvasive manner, could aid translation of these 3D models into HTS applications.
dc.language.isoeng
dc.relation.ispartofJournal of Biophotonicsen
dc.rightsCopyright © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1002/jbio.201800201en
dc.subjectMulticellular tumour spheroidsen
dc.subjectRamanen
dc.subjectCanceren
dc.subjectDrug screeningen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectNDASen
dc.subject.lccRC0254en
dc.titleRaman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporineen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews.Sir James Mackenzie Institute for Early Diagnosisen
dc.identifier.doihttps://doi.org/10.1002/jbio.201800201
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-01-09


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