Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorHudson, Clare
dc.contributor.authorEsposito, Rosaria
dc.contributor.authorPalladino, Antonio
dc.contributor.authorStaiano, Leopoldo
dc.contributor.authorFerrier, David
dc.contributor.authorFaure, Emmanuel
dc.contributor.authorLemaire, Patrick
dc.contributor.authorYasuo, Hitoyoshi
dc.contributor.authorSpagnuolo, Antonietta
dc.date.accessioned2019-12-21T00:36:29Z
dc.date.available2019-12-21T00:36:29Z
dc.date.issued2019-04-15
dc.identifier.citationHudson , C , Esposito , R , Palladino , A , Staiano , L , Ferrier , D , Faure , E , Lemaire , P , Yasuo , H & Spagnuolo , A 2019 , ' Transcriptional regulation of the Ciona Gsx gene in the neural plate ' , Developmental Biology , vol. 448 , no. 2 , pp. 88-100 . https://doi.org/10.1016/j.ydbio.2018.12.013en
dc.identifier.issn0012-1606
dc.identifier.otherPURE: 257259402
dc.identifier.otherPURE UUID: 04cc52ec-32ab-48b0-a510-588b209bf37d
dc.identifier.otherRIS: urn:0223F2C437DE6A55615DE1C092B4F277
dc.identifier.otherScopus: 85058799388
dc.identifier.otherORCID: /0000-0003-3247-6233/work/52572454
dc.identifier.otherWOS: 000466825700003
dc.identifier.urihttps://hdl.handle.net/10023/19189
dc.descriptionWork by R. E. in the laboratory of H. Y. was supported by a European Molecular Biology Organisation (EMBO) short term fellowship (ASTF 534–2014). Work by P. L. and E. F. was supported by CNRS and the Agence Nationale de la Recherche (ANR-13-BSV2–0011-01,TED; ANR-08-BLAN-0067, Chor-Evo-Net). Work by R. E., A. P. and L. S. in the laboratory of A. S. was supported by SZN PhD fellowships. The group of D. E. K. F. is supported by the Leverhulme Trust (RPG-2016-351). Isolation of the Gsx containing cosmid was conducted in the laboratory of Peter Holland and supported by the Biotechnology and Biological Sciences Research Council (BBSRC) (no. G09218).en
dc.description.abstractThe ascidian neural plate consists of a defined number of identifiable cells organized in a grid of rows and columns, representing a useful model to investigate the molecular mechanisms controlling neural patterning in chordates. Distinct anterior brain lineages are specified via unique combinatorial inputs of signalling pathways with Nodal and Delta-Notch signals patterning along the medial-lateral axis and FGF/MEK/ERK signals patterning along the anterior-posterior axis of the neural plate. The Ciona Gsx gene is specifically expressed in the a9.33 cells in the row III/column 2 position of anterior brain lineages, characterised by a combinatorial input of Nodal-OFF, Notch-ON and FGF-ON. Here, we identify the minimal cis-regulatory element (CRE) of 376 bp, which can recapitulate the early activation of Gsx. We show that this minimal CRE responds in the same way as the endogenous Gsx gene to manipulation of FGF- and Notch-signalling pathways and to overexpression of Snail, a mediator of Nodal signals, and Six3/6, which is required to demarcate the anterior boundary of Gsx expression at the late neurula stage. We reveal that sequences proximal to the transcription start site include a temporal regulatory element required for the precise transcriptional onset of gene expression. We conclude that sufficient spatial and temporal information for Gsx expression is integrated in 376 bp of non-coding cis-regulatory sequences.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofDevelopmental Biologyen
dc.rights© 2018, Elsevier Inc. This work has been made available online in accordance with the publisher's policies. This is the author created accepted version manuscript following peer review and as such may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.ydbio.2018.12.013en
dc.subjectAscidianen
dc.subjectCionaen
dc.subjectGsxen
dc.subjectTranscriptional regulationen
dc.subjectPromoteren
dc.subjectNeural patterningen
dc.subjectPara-hoxen
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subject.lccQH301en
dc.titleTranscriptional regulation of the Ciona Gsx gene in the neural plateen
dc.typeJournal articleen
dc.contributor.sponsorThe Leverhulme Trusten
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.identifier.doihttps://doi.org/10.1016/j.ydbio.2018.12.013
dc.description.statusPeer revieweden
dc.date.embargoedUntil2019-12-21
dc.identifier.grantnumberORPG-5203en


This item appears in the following Collection(s)

Show simple item record