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dc.contributor.authorBimba, John S
dc.contributor.authorLawson, Lovett
dc.contributor.authorKontogianni, Konstantina
dc.contributor.authorEdwards, Thomas
dc.contributor.authorEkpenyong, Bassey Emanna
dc.contributor.authorDodd, James
dc.contributor.authorAdams, Emily R
dc.contributor.authorSloan, Derek James
dc.contributor.authorCresswell, Jacob
dc.contributor.authorDominguez, Jose
dc.contributor.authorCuevas, Luis E
dc.identifier.citationBimba , J S , Lawson , L , Kontogianni , K , Edwards , T , Ekpenyong , B E , Dodd , J , Adams , E R , Sloan , D J , Cresswell , J , Dominguez , J & Cuevas , L E 2019 , ' PrimeStore MTM and OMNIgene sputum for the preservation of sputum for Xpert MTB/RIF testing in Nigeria ' , Journal of Clinical Medicine , vol. 8 , no. 12 , 2146 .
dc.identifier.otherPURE: 264039065
dc.identifier.otherPURE UUID: c4180d96-d1dc-4109-930a-427fc8d4b019
dc.identifier.otherWOS: 000506640400116
dc.identifier.otherScopus: 85094943098
dc.identifier.otherORCID: /0000-0002-7888-5449/work/65702635
dc.descriptionThis research was funded by the European and Developing Countries Clinical Trial Partnership (EDCTP), grant number DRIA2014-309, and its co-funders were the Medical Research Council (MRC), UK, Instituto de Salud Carlos III (ISCIII Spain) and Global Affairs Canada, through the TB REACH Initiative of the Stop TB Partnership, Wave 5, project number STBP/TBREACH//GSA/W5-07.en
dc.description.abstractBackground: Xpert MTB/RIF (GX) for tuberculosis (TB) diagnosis is often located in reference laboratories, and sputum needs to be transported using a cold chain. Transport media to preserve sputum are available, but performance data under programmatic conditions are limited. Methods: Sputum samples were collected from patients with presumptive TB in Nigeria. One sputum was transported in a cold chain, tested immediately with GX and cultured. One sputum was swabbed and stored in PrimeStore-Molecular-Transport-Medium (Primestore), and the remainder was stored in OMNIGene-sputum (Omnigene), kept for seven days and tested with GX. Results: Of 248 patients, 63 were fresh-sputum culture-positive and 56 GX-positive (sensitivity 88.9%, 95% CI: 78.4–95.4%). Four of 185 culture-negative patients were GX-positive (specificity 97.8%, 94.6–99.4%). Omnigene GX and Primestore GX were positive in 56/62 (90.3%, 80.1–96.4%) and 49/62 (79.0%, 66.8–88.3%) culture-positive, respectively, and 1/185 (99.5%, 97.0–100.0%) and 3/185 (98.4%, 95.3–99.7%) were culture-negative patients. 14 Human Immunodeficiency Virus (HIV)-infected and 44 HIV-uninfected patients were culture-positive. Omnigene and Primestore detected 12/14 (85.7%, 57.2–98.2%) and 5/14 (35.7%, 12.8–64.9%) HIV-infected and 41/44 (93.2%, 81.3–98.6%) HIV-uninfected culture-positive patients. Interpretation : Omnigene stored and fresh sputum samples had similar GX results. The GX results of Primestore-stored samples were similar to those found in the fresh sputum of non-HIV infected patients, but GX-positivity was lower in HIV-infected patients. This was likely due to the lower amount of bacilli collected by the swab and transferred to PrimeStore.
dc.relation.ispartofJournal of Clinical Medicineen
dc.rightsCopyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
dc.subjectXpert MTB/RIFen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titlePrimeStore MTM and OMNIgene sputum for the preservation of sputum for Xpert MTB/RIF testing in Nigeriaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.description.statusPeer revieweden

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