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Probing neural tissue with airy light-sheet microscopy : investigation of imaging performance at depth within turbid media
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dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | McCluskey, Kaley | |
dc.contributor.author | Aggarwal, Sanya | |
dc.contributor.author | Tello, Javier A. | |
dc.contributor.author | Dholakia, Kishan | |
dc.contributor.editor | Brown, Thomas G. | |
dc.contributor.editor | Cogswell, Carol J. | |
dc.contributor.editor | Wilson, Tony | |
dc.date.accessioned | 2019-11-15T09:30:08Z | |
dc.date.available | 2019-11-15T09:30:08Z | |
dc.date.issued | 2017-02-17 | |
dc.identifier | 262481486 | |
dc.identifier | 5bfb37ab-a4e5-409f-b335-4a2414c8722f | |
dc.identifier | 85020252169 | |
dc.identifier | 000405790300006 | |
dc.identifier.citation | Nylk , J , McCluskey , K , Aggarwal , S , Tello , J A & Dholakia , K 2017 , Probing neural tissue with airy light-sheet microscopy : investigation of imaging performance at depth within turbid media . in T G Brown , C J Cogswell & T Wilson (eds) , Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXIV . , 100700B , Proceedings of SPIE , vol. 10070 , SPIE , pp. 1-7 , Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV 2017 , San Francisco , California , United States , 30/01/17 . https://doi.org/10.1117/12.2251921 | en |
dc.identifier.citation | conference | en |
dc.identifier.isbn | 9781510605817 | |
dc.identifier.isbn | 9781510605824 | |
dc.identifier.issn | 1605-7422 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/64034482 | |
dc.identifier.other | ORCID: /0000-0001-6637-2155/work/64034517 | |
dc.identifier.uri | https://hdl.handle.net/10023/18933 | |
dc.description | Funding: UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1 (KD), the 'BRAINS' 600th anniversary appeal, and Dr. E. Killick; The Northwood Trust and The RS Macdonald Charitable Trust (JAT); Royal Society Leverhulme Trust Senior Fellowship (KD). | en |
dc.description.abstract | Light-sheet microscopy (LSM) has received great interest for fluorescent imaging applications in biomedicine as it facilitates three-dimensional visualisation of large sample volumes with high spatiotemporal resolution whilst minimising irradiation of, and photo-damage to the specimen. Despite these advantages, LSM can only visualize superficial layers of turbid tissues, such as mammalian neural tissue. Propagation-invariant light modes have played a key role in the development of high-resolution LSM techniques as they overcome the natural divergence of a Gaussian beam, enabling uniform and thin light-sheets over large distances. Most notably, Bessel and Airy beam-based light-sheet imaging modalities have been demonstrated. In the single-photon excitation regime and in lightly scattering specimens, Airy-LSM has given competitive performance with advanced Bessel-LSM techniques. Airy and Bessel beams share the property of self-healing, the ability of the beam to regenerate its transverse beam profile after propagation around an obstacle. Bessel-LSM techniques have been shown to increase the penetration-depth of the illumination into turbid specimens but this effect has been understudied in biologically relevant tissues, particularly for Airy beams. It is expected that Airy-LSM will give a similar enhancement over Gaussian-LSM. In this paper, we report on the comparison of Airy-LSM and Gaussian-LSM imaging modalities within cleared and non-cleared mouse brain tissue. In particular, we examine image quality versus tissue depth by quantitative spatial Fourier analysis of neural structures in virally transduced fluorescent tissue sections, showing a three-fold enhancement at 50 μm depth into non-cleared tissue with Airy-LSM. Complimentary analysis is performed by resolution measurements in bead-injected tissue sections. | |
dc.format.extent | 7 | |
dc.format.extent | 614015 | |
dc.language.iso | eng | |
dc.publisher | SPIE | |
dc.relation.ispartof | Three-Dimensional and Multidimensional Microscopy | en |
dc.relation.ispartofseries | Proceedings of SPIE | en |
dc.subject | Light-sheet microscopy | en |
dc.subject | LSM | en |
dc.subject | Airy beam | en |
dc.subject | Tissue imaging | en |
dc.subject | Turbid media | en |
dc.subject | Neuroscience | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | Biomaterials | en |
dc.subject | Electronic, Optical and Magnetic Materials | en |
dc.subject | Radiology Nuclear Medicine and imaging | en |
dc.subject | Atomic and Molecular Physics, and Optics | en |
dc.subject | NS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | RC0321 | en |
dc.title | Probing neural tissue with airy light-sheet microscopy : investigation of imaging performance at depth within turbid media | en |
dc.type | Conference item | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1117/12.2251921 | |
dc.identifier.grantnumber | EP/J01771X/1 | en |
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