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Three-photon light-sheet fluorescence microscopy

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dc.contributor.authorEscobet Montalban, Adria
dc.contributor.authorGasparoli, Federico M.
dc.contributor.authorNylk, Jonathan
dc.contributor.authorLiu, Pengfei
dc.contributor.authorYang, Zhengyi
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2019-11-01T00:36:22Z
dc.date.available2019-11-01T00:36:22Z
dc.date.issued2018-11-01
dc.identifier.citationEscobet Montalban , A , Gasparoli , F M , Nylk , J , Liu , P , Yang , Z & Dholakia , K 2018 , ' Three-photon light-sheet fluorescence microscopy ' , Optics Letters , vol. 43 , no. 21 , pp. 5484-5487 . https://doi.org/10.1364/OL.43.005484en
dc.identifier.issn0146-9592
dc.identifier.otherPURE: 256162237
dc.identifier.otherPURE UUID: e0adbc6d-b5ed-4818-bb3b-69a7f3b481ad
dc.identifier.otherScopus: 85056096407
dc.identifier.otherORCID: /0000-0002-2977-4929/work/50167381
dc.identifier.otherWOS: 000448939000080
dc.identifier.urihttps://hdl.handle.net/10023/18816
dc.descriptionThis work has received funding from the European Union’s Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BEOPTICAL) under grant agreement no. 675512 and the UK Engineering and Physical Sciences Research Council (grants EP/P030017/1 and EP/R004854/1).en
dc.description.abstractWe present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime enhancing its penetration depth. Our present study uses a standard conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofOptics Lettersen
dc.rightsCopyright © 2018 Optical Society of America. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://doi.org/10.1364/OL.43.005484en
dc.subjectQC Physicsen
dc.subjectDASen
dc.subject.lccQCen
dc.titleThree-photon light-sheet fluorescence microscopyen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEPSRCen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1364/OL.43.005484
dc.description.statusPeer revieweden
dc.date.embargoedUntil2019-11-01
dc.identifier.urlhttps://doi.org/10.1101/323790en
dc.identifier.grantnumber675512en
dc.identifier.grantnumberEP/P030017/1en
dc.identifier.grantnumberEP/R004854/1en


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