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dc.contributor.authorDebierre-Grockiego, Françoise
dc.contributor.authorSmith, Terry K.
dc.contributor.authorDelbecq, Stéphane
dc.contributor.authorDucournau, Céline
dc.contributor.authorLantier, Louis
dc.contributor.authorSchmidt, Jörg
dc.contributor.authorBrès, Virginie
dc.contributor.authorDimier-Poisson, Isabelle
dc.contributor.authorSchwarz, Ralph T.
dc.contributor.authorCornillot, Emmanuel
dc.date.accessioned2019-10-31T16:30:12Z
dc.date.available2019-10-31T16:30:12Z
dc.date.issued2019-12
dc.identifier.citationDebierre-Grockiego , F , Smith , T K , Delbecq , S , Ducournau , C , Lantier , L , Schmidt , J , Brès , V , Dimier-Poisson , I , Schwarz , R T & Cornillot , E 2019 , ' Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cells ' , Biochimie , vol. 167 , pp. 135-144 . https://doi.org/10.1016/j.biochi.2019.09.007en
dc.identifier.issn0300-9084
dc.identifier.otherPURE: 262577344
dc.identifier.otherPURE UUID: e0de7072-ebe8-44a5-8012-e7f7f65ad6c4
dc.identifier.othercrossref: 10.1016/j.biochi.2019.09.007
dc.identifier.otherScopus: 85072926229
dc.identifier.otherWOS: 000496992000013
dc.identifier.urihttp://hdl.handle.net/10023/18813
dc.descriptionThis work was supported by the the University of Tours (to IDP and FDG), the University of Montpellier (to SD and EC), the Deutsche Forschungsgemeinschaft (to RTS), the Wellcome Trust project grant 093228 (to TKS) and the Campus France/DAAD PHC PROCOPE 24931RE (to RTS and EC). The funding source has no involvement in the conduct of the research and preparation of the article.en
dc.description.abstractGlycosylphosphatidylinositols (GPIs) are glycolipids described as toxins of protozoan parasites due to their inflammatory properties in mammalian hosts characterized by the production of interleukin (IL)-1, IL-12 and tumor necrosis factor (TNF)-α. In the present work, we studied the cytokines produced by antigen presenting cells in response to ten different GPI species extracted from Babesia divergens, responsible for babesiosis. Interestingly, B. divergens GPIs induced the production of anti-inflammatory cytokines (IL-2, IL-5) and of the regulatory cytokine IL-10 by macrophages and dendritic cells. In contrast to all protozoan GPIs studied until now, GPIs from B. divergens did not stimulate the production of TNF-α and IL-12, leading to a unique Th1/Th2 profile. Analysis of the carbohydrate composition of the B. divergens GPIs indicated that the di-mannose structure was different from the evolutionary conserved tri-mannose structure, which might explain the particular cytokine profile they induce. Expression of major histocompatibility complex (MHC) molecules on dendritic cells and apoptosis of mouse peritoneal cells were also analysed. B. divergens GPIs did not change expression of MHC class I, but decreased expression of MHC class II at the cell surface, while GPIs slightly increased the percentages of apoptotic cells. During pathogenesis of babesiosis, the inflammation-coagulation auto-amplification loop can lead to thrombosis and the effect of GPIs on coagulation parameters was investigated. Incubation of B. divergens GPIs with rat plasma ex vivo led to increase of fibrinogen levels and to prolonged activated partial thromboplastin time, suggesting a direct modulation of the extrinsic coagulation pathway by GPIs.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofBiochimieen
dc.rightsCopyright © 2019 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectAntigen presentationen
dc.subjectCoagulationen
dc.subjectGlycosylphosphatidylinositolen
dc.subjectInterleukinen
dc.subjectMajor histocompatibility complexen
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectNDASen
dc.subject.lccQDen
dc.subject.lccQH301en
dc.subject.lccRC0254en
dc.titleBabesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cellsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1016/j.biochi.2019.09.007
dc.description.statusPeer revieweden


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