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Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle
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dc.contributor.author | Holt, Ian | |
dc.contributor.author | Fuller, Heidi R. | |
dc.contributor.author | Lam, Le Thanh | |
dc.contributor.author | Sewry, Caroline A. | |
dc.contributor.author | Shirran, Sally L. | |
dc.contributor.author | Zhang, Qiuping | |
dc.contributor.author | Shanahan, Catherine M. | |
dc.contributor.author | Morris, Glenn E. | |
dc.date.accessioned | 2019-10-11T11:30:11Z | |
dc.date.available | 2019-10-11T11:30:11Z | |
dc.date.issued | 2019-10-02 | |
dc.identifier | 261743652 | |
dc.identifier | 6db20b43-c0f6-4a93-aae3-02acd36fe47a | |
dc.identifier | 85072909439 | |
dc.identifier | 000488481500065 | |
dc.identifier.citation | Holt , I , Fuller , H R , Lam , L T , Sewry , C A , Shirran , S L , Zhang , Q , Shanahan , C M & Morris , G E 2019 , ' Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle ' , Scientific Reports , vol. 9 , 14202 . https://doi.org/10.1038/s41598-019-50728-6 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | RIS: urn:6BCB686D0E48FD947DF22F56715DBF6C | |
dc.identifier.other | RIS: Holt2019 | |
dc.identifier.other | ORCID: /0000-0003-3516-3507/work/63044667 | |
dc.identifier.uri | https://hdl.handle.net/10023/18646 | |
dc.description | This project was supported by grants from the British Heart Foundation (PG/11/71/29091 and PG/16/68/31991) and the Orthopaedic Institute Ltd., (RJAH Orthopaedic Hospital, Oswestry, UK). | en |
dc.description.abstract | Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane. | |
dc.format.extent | 12 | |
dc.format.extent | 4271234 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | DAS | en |
dc.subject.lcc | RC0321 | en |
dc.title | Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1038/s41598-019-50728-6 | |
dc.description.status | Peer reviewed | en |
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