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Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle
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dc.contributor.author | Holt, Ian | |
dc.contributor.author | Fuller, Heidi R. | |
dc.contributor.author | Lam, Le Thanh | |
dc.contributor.author | Sewry, Caroline A. | |
dc.contributor.author | Shirran, Sally L. | |
dc.contributor.author | Zhang, Qiuping | |
dc.contributor.author | Shanahan, Catherine M. | |
dc.contributor.author | Morris, Glenn E. | |
dc.date.accessioned | 2019-10-11T11:30:11Z | |
dc.date.available | 2019-10-11T11:30:11Z | |
dc.date.issued | 2019-10-02 | |
dc.identifier.citation | Holt , I , Fuller , H R , Lam , L T , Sewry , C A , Shirran , S L , Zhang , Q , Shanahan , C M & Morris , G E 2019 , ' Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle ' , Scientific Reports , vol. 9 , 14202 . https://doi.org/10.1038/s41598-019-50728-6 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | PURE: 261743652 | |
dc.identifier.other | PURE UUID: 6db20b43-c0f6-4a93-aae3-02acd36fe47a | |
dc.identifier.other | RIS: urn:6BCB686D0E48FD947DF22F56715DBF6C | |
dc.identifier.other | RIS: Holt2019 | |
dc.identifier.other | ORCID: /0000-0003-3516-3507/work/63044667 | |
dc.identifier.other | Scopus: 85072909439 | |
dc.identifier.other | WOS: 000488481500065 | |
dc.identifier.uri | https://hdl.handle.net/10023/18646 | |
dc.description | This project was supported by grants from the British Heart Foundation (PG/11/71/29091 and PG/16/68/31991) and the Orthopaedic Institute Ltd., (RJAH Orthopaedic Hospital, Oswestry, UK). | en |
dc.description.abstract | Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane. | |
dc.format.extent | 12 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.rights | Copyright © The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | DAS | en |
dc.subject.lcc | RC0321 | en |
dc.title | Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1038/s41598-019-50728-6 | |
dc.description.status | Peer reviewed | en |
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