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dc.contributor.authorGrüschow, Sabine
dc.contributor.authorAthukoralage, Januka S
dc.contributor.authorGraham, Shirley
dc.contributor.authorHoogeboom, Tess
dc.contributor.authorWhite, Malcolm F
dc.date.accessioned2019-10-09T16:30:03Z
dc.date.available2019-10-09T16:30:03Z
dc.date.issued2019-09-26
dc.identifier.citationGrüschow , S , Athukoralage , J S , Graham , S , Hoogeboom , T & White , M F 2019 , ' Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence ' , Nucleic Acids Research , vol. 47 , no. 17 , pp. 9259-9270 . https://doi.org/10.1093/nar/gkz676en
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 261681537
dc.identifier.otherPURE UUID: 1c3d13db-4e42-4972-8c5e-d413cb8ce257
dc.identifier.otherBibtex: 10.1093/nar/gkz676
dc.identifier.otherScopus: 85072588750
dc.identifier.otherORCID: /0000-0003-1543-9342/work/63044332
dc.identifier.otherORCID: /0000-0002-1666-0180/work/63046273
dc.identifier.urihttp://hdl.handle.net/10023/18633
dc.descriptionRoyal Society Challenge Grant [REF: CH160014 to M.F.W.]; Biotechnology and Biological Sciences Research Council [REF: BB/S000313/1 to M.F.W.]. Funding for open access charge: Institutional Block Grant.en
dc.description.abstractThe CRISPR system provides adaptive immunity against mobile genetic elements (MGE) in prokaryotes. In type III CRISPR systems, an effector complex programmed by CRISPR RNA detects invading RNA, triggering a multi-layered defence that includes target RNA cleavage, licencing of an HD DNA nuclease domain and synthesis of cyclic oligoadenylate (cOA) molecules. cOA activates the Csx1/Csm6 family of effectors, which degrade RNA non-specifically to enhance immunity. Type III systems are found in diverse archaea and bacteria, including the human pathogen Mycobacterium tuberculosis. Here, we report a comprehensive analysis of the in vitro and in vivo activities of the type III-A M. tuberculosis CRISPR system. We demonstrate that immunity against MGE may be achieved predominantly via a cyclic hexa-adenylate (cA6) signalling pathway and the ribonuclease Csm6, rather than through DNA cleavage by the HD domain. Furthermore, we show for the first time that a type III CRISPR system can be reprogrammed by replacing the effector protein, which may be relevant for maintenance of immunity in response to pressure from viral anti-CRISPRs. These observations demonstrate that M. tuberculosis has a fully-functioning CRISPR interference system that generates a range of cyclic and linear oligonucleotides of known and unknown functions, potentiating fundamental and applied studies.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.rightsCopyright © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectNDASen
dc.subject.lccRMen
dc.titleCyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defenceen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/nar/gkz676
dc.description.statusPeer revieweden


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