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dc.contributor.authorLooseley, Mark E.
dc.contributor.authorBayer, Micha
dc.contributor.authorBull, Hazel
dc.contributor.authorRamsay, Luke
dc.contributor.authorThomas, William
dc.contributor.authorBooth, Allan
dc.contributor.authorDe La Fuente Canto, Carla
dc.contributor.authorMorris, Jenny
dc.contributor.authorHedley, Pete E.
dc.contributor.authorRussell, Joanne
dc.date.accessioned2019-09-05T14:30:05Z
dc.date.available2019-09-05T14:30:05Z
dc.date.issued2017-09-12
dc.identifier.citationLooseley , M E , Bayer , M , Bull , H , Ramsay , L , Thomas , W , Booth , A , De La Fuente Canto , C , Morris , J , Hedley , P E & Russell , J 2017 , ' Association mapping of diastatic power in UK winter and spring barley by exome sequencing of phenotypically contrasting variety sets ' , Frontiers in Plant Science , vol. 8 , 1566 . https://doi.org/10.3389/fpls.2017.01566en
dc.identifier.issn1664-462X
dc.identifier.otherPURE: 261029559
dc.identifier.otherPURE UUID: 2689521b-96a2-4158-b4cf-62f043c916b9
dc.identifier.otherWOS: 000410270800001
dc.identifier.otherScopus: 85030854821
dc.identifier.urihttp://hdl.handle.net/10023/18429
dc.description.abstractDiastatic Power (DP) is an important quality trait for malt used in adjunct brewing and distilling. Substantial genetic variation for DP exists within UK elite barley cultivars, but breeding progress has been slow due to the limited demand, compared to the overall barley market, and difficulties in assessing DP. Estimates of DP (taken from recommended and national list trials between 1994 and 2012) from a collection of UK elite winter and spring varieties were used to identify contrasting sets of high and low DP varieties. DNA samples were pooled within sets and exome capture sequencing performed. Allele frequency estimates of Single Nucleotide Polymorphisms (SNPs) identified from the sequencing were used to identify genomic locations associated with differences in DP. Individual genotypes were generated from a set of custom KASP assays, both within sets and in a wider germplasm collection, to validate allele frequency estimates and marker associations with DP. QTL identified regions previously linked to variation in DP as well as novel associations. QTL colocalised with a number of genes annotated as having a diastase related function. Results indicate that winter barley is more genetically diverse for genes influencing DP. The marker assays produced by this work represent a resource that is available for immediate use by barley breeders in the production of new high DP varieties.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofFrontiers in Plant Scienceen
dc.rightsCopyright © 2017 Looseley, Bayer, Bull, Ramsay, Thomas, Booth, De La Fuente Canto, Morris, Hedley and Russell. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectBarleyen
dc.subjectExome captureen
dc.subjectMalting qualityen
dc.subjectDiastatic poweren
dc.subjectQTL mappingen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleAssociation mapping of diastatic power in UK winter and spring barley by exome sequencing of phenotypically contrasting variety setsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.3389/fpls.2017.01566
dc.description.statusPeer revieweden


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