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dc.contributor.authorMuh, Fauzi
dc.contributor.authorLee, Seong-Kyun
dc.contributor.authorHoque, Mohammad Rafiul
dc.contributor.authorHan, Jin-Hee
dc.contributor.authorPark, Ji-Hoon
dc.contributor.authorFirdaus, Egy Rahman
dc.contributor.authorMoon, Robert W.
dc.contributor.authorLau, Yee Ling
dc.contributor.authorHan, Eun-Taek
dc.date.accessioned2019-07-18T11:30:08Z
dc.date.available2019-07-18T11:30:08Z
dc.date.issued2018-07-27
dc.identifier259673185
dc.identifierb39ef903-81ff-4911-a345-f19b9f363142
dc.identifier000440181600001
dc.identifier85050721633
dc.identifier.citationMuh , F , Lee , S-K , Hoque , M R , Han , J-H , Park , J-H , Firdaus , E R , Moon , R W , Lau , Y L & Han , E-T 2018 , ' In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain ' , Malaria Journal , vol. 17 , 272 . https://doi.org/10.1186/s12936-018-2420-4en
dc.identifier.issn1475-2875
dc.identifier.otherORCID: /0000-0002-3966-9110/work/59698773
dc.identifier.urihttps://hdl.handle.net/10023/18124
dc.description.abstractBackground: The rapid process of malaria erythrocyte invasion involves ligand-receptor interactions. Inducing antibodies against specific ligands or receptors that abrogate the invasion process is a key challenge for blood stage vaccine development. However, few candidates were reported and remain to be validated for the discovery of new vaccine candidates in Plasmodium knowlesi. Methods: In order to investigate the efficacy of pre-clinical vaccine candidates in P. knowlesi-infected human cases, this study describes an in vitro invasion inhibition assay, using a P. knowlesi strain adapted to in vitro growth in human erythrocytes, PkA1-H. 1. Recombinant proteins of P. knowlesi Duffy binding protein alpha (PkDBPα) and apical membrane antigen 1 (PkAMA1) were produced in Escherichia coli system and rabbit antibodies were generated from immune animals. Results: PkDBPα and PkAMA1 recombinant proteins were expressed as insoluble and produced as a functional refolded form for this study. Antibodies against PkDBPα and PkAMA1 specifically recognized recombinant proteins and native parasite proteins in schizont-stage parasites on the merozoite organelles. Single and combination of anti-PkDBPα and anti-PkAMA1 antibodies elicited strong growth inhibitory effects on the parasite in concentrationdependent manner. Meanwhile, IgG prevalence of PkDBPα and PkAMA1 were observed in 13.0 and 46.7% in human clinical patients, respectively. Conclusion: These data provide support for the validation of in vitro growth inhibition assay using antibodies of DBPα and AMA1 in human-adapted P. knowlesi parasite PkA1-H. 1 strain.
dc.format.extent11
dc.format.extent1761760
dc.language.isoeng
dc.relation.ispartofMalaria Journalen
dc.subjectPlasmodium knowlesien
dc.subjectZoonotic malariaen
dc.subjectPkDBP alphaen
dc.subjectPkAMA1en
dc.subjectInvasionen
dc.subjectInhibitionen
dc.subjectQH301 Biologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectNDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQH301en
dc.subject.lccRMen
dc.titleIn vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strainen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.identifier.doi10.1186/s12936-018-2420-4
dc.description.statusPeer revieweden


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